Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)

Cotton (<em>Gossypium hirsutum</em> L.) is a source of edible oil and fatty acids (FAs). In this study, embryos’ explants were cultured in MS medium with different concentration of  plant growth regulators (phytohormones) include BA, IAA, IBA, 2, 4- D and NAA, to finding the relationship...

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Main Authors: Babak Delnavaz Hashemloian, Azra Ataei Azimi
Format: Article
Language:English
Published: Genetics and Agricultural Biotechnology Institute of Tabarestan 2017-12-01
Series:Journal of Plant Molecular Breeding
Subjects:
Online Access:http://www.jpmb-gabit.ir/article_31439_815f04c6d505be02c995d6456a795b93.pdf
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author Babak Delnavaz Hashemloian
Azra Ataei Azimi
author_facet Babak Delnavaz Hashemloian
Azra Ataei Azimi
author_sort Babak Delnavaz Hashemloian
collection DOAJ
description Cotton (<em>Gossypium hirsutum</em> L.) is a source of edible oil and fatty acids (FAs). In this study, embryos’ explants were cultured in MS medium with different concentration of  plant growth regulators (phytohormones) include BA, IAA, IBA, 2, 4- D and NAA, to finding the relationship of hormone and organogenesis with oil and FAs content. Oil was extracted with chloroform and methanol. FAs of oil were esterificated and methylated for GC-MS analysis. The seeds<strong> and shoots oil were 36.1 and 4.5%. Oil of </strong>regenerated shoots <strong>(<strong>33.3%</strong>) <strong>in T2 with 0.25 BA and 5 mgl<sup>-1</sup> IAA was higher than to seeds, shoots and all regenerated organs.</strong></strong>Unsaturated FAs increased in some treatments depending on the type of phytohormone and organogenesis.  Amounts of all FAs of regenerated root of T4 in BA/IBA were lesser than to other samples. Our results showed that Sahel cultivar of cotton can synthesize 12 types of FAs, but some of those FAs are made under certain conditions depending on the type and concentration of phytohormones and organogenesis.
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spelling doaj.art-12265d260842477db7fb7151db0bdb3a2022-12-21T18:57:09ZengGenetics and Agricultural Biotechnology Institute of TabarestanJournal of Plant Molecular Breeding2322-33322322-50922017-12-0152202610.22058/jpmb.2018.78726.115531439Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)Babak Delnavaz Hashemloian0Azra Ataei Azimi1Department of Biology, Faculty of Agriculture, Saveh branch, Islamic Azad University, Saveh, IranDepartment of Biology, Faculty of Agriculture, Saveh branch, Islamic Azad University, Saveh, IranCotton (<em>Gossypium hirsutum</em> L.) is a source of edible oil and fatty acids (FAs). In this study, embryos’ explants were cultured in MS medium with different concentration of  plant growth regulators (phytohormones) include BA, IAA, IBA, 2, 4- D and NAA, to finding the relationship of hormone and organogenesis with oil and FAs content. Oil was extracted with chloroform and methanol. FAs of oil were esterificated and methylated for GC-MS analysis. The seeds<strong> and shoots oil were 36.1 and 4.5%. Oil of </strong>regenerated shoots <strong>(<strong>33.3%</strong>) <strong>in T2 with 0.25 BA and 5 mgl<sup>-1</sup> IAA was higher than to seeds, shoots and all regenerated organs.</strong></strong>Unsaturated FAs increased in some treatments depending on the type of phytohormone and organogenesis.  Amounts of all FAs of regenerated root of T4 in BA/IBA were lesser than to other samples. Our results showed that Sahel cultivar of cotton can synthesize 12 types of FAs, but some of those FAs are made under certain conditions depending on the type and concentration of phytohormones and organogenesis.http://www.jpmb-gabit.ir/article_31439_815f04c6d505be02c995d6456a795b93.pdfphytohormoneorganogenesisGC-MSSaponification, Regeneration
spellingShingle Babak Delnavaz Hashemloian
Azra Ataei Azimi
Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
Journal of Plant Molecular Breeding
phytohormone
organogenesis
GC-MS
Saponification, Regeneration
title Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
title_full Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
title_fullStr Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
title_full_unstemmed Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
title_short Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)
title_sort comparison of oil and fatty acid composition of seed shoot and in vitro regenerated organs of cotton gossypium hirsutum l var sahel
topic phytohormone
organogenesis
GC-MS
Saponification, Regeneration
url http://www.jpmb-gabit.ir/article_31439_815f04c6d505be02c995d6456a795b93.pdf
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