Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads
STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetical...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122003403 |
| _version_ | 1797956219027062784 |
|---|---|
| author | Albert Blanch-Asensio Babet van der Vaart Mariana Vinagre Eline Groen Christiaan Arendzen Christian Freund Niels Geijsen Christine L. Mummery Richard P. Davis |
| author_facet | Albert Blanch-Asensio Babet van der Vaart Mariana Vinagre Eline Groen Christiaan Arendzen Christian Freund Niels Geijsen Christine L. Mummery Richard P. Davis |
| author_sort | Albert Blanch-Asensio |
| collection | DOAJ |
| description | STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications. |
| first_indexed | 2024-04-10T23:45:30Z |
| format | Article |
| id | doaj.art-1226a2ec50804aaa818af1245a0a9948 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-10T23:45:30Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-1226a2ec50804aaa818af1245a0a99482023-01-11T04:28:27ZengElsevierStem Cell Research1873-50612023-02-0166102991Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloadsAlbert Blanch-Asensio0Babet van der Vaart1Mariana Vinagre2Eline Groen3Christiaan Arendzen4Christian Freund5Niels Geijsen6Christine L. Mummery7Richard P. Davis8Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; LUMC hiPSC Hotel, Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; LUMC hiPSC Hotel, Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; LUMC hiPSC Hotel, Department of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the Netherlands; Department of Applied Stem Cell Technologies, University of Twente, 7500AE Enschede, the NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, 2300RC Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 2300RC Leiden, the Netherlands; Corresponding author.STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications.http://www.sciencedirect.com/science/article/pii/S1873506122003403 |
| spellingShingle | Albert Blanch-Asensio Babet van der Vaart Mariana Vinagre Eline Groen Christiaan Arendzen Christian Freund Niels Geijsen Christine L. Mummery Richard P. Davis Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads Stem Cell Research |
| title | Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads |
| title_full | Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads |
| title_fullStr | Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads |
| title_full_unstemmed | Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads |
| title_short | Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads |
| title_sort | generation of aavs1 and clybl straight in v2 acceptor human ipsc lines for integrating dna payloads |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122003403 |
| work_keys_str_mv | AT albertblanchasensio generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT babetvandervaart generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT marianavinagre generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT elinegroen generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT christiaanarendzen generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT christianfreund generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT nielsgeijsen generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT christinelmummery generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads AT richardpdavis generationofaavs1andclyblstraightinv2acceptorhumanipsclinesforintegratingdnapayloads |