Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads
STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2–3 weeks) generation of genetical...
Main Authors: | Albert Blanch-Asensio, Babet van der Vaart, Mariana Vinagre, Eline Groen, Christiaan Arendzen, Christian Freund, Niels Geijsen, Christine L. Mummery, Richard P. Davis |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-02-01
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Series: | Stem Cell Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122003403 |
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