Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat

Serine hydroxymethyltransferase (SHMT) plays a pivotal role in cellular one-carbon, photorespiration pathways and it influences the resistance to biotic and abiotic stresses. However, the function of SHMT proteins in wheat remains largely unexplored. In the present study, SHMT genes in five Triticea...

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Main Authors: Ping Hu, Puwen Song, Jun Xu, Qichao Wei, Ye Tao, Yueming Ren, Yongang Yu, Dongxiao Li, Haiyan Hu, Chengwei Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-02-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.847087/full
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author Ping Hu
Puwen Song
Jun Xu
Qichao Wei
Ye Tao
Ye Tao
Yueming Ren
Yongang Yu
Dongxiao Li
Haiyan Hu
Chengwei Li
Chengwei Li
author_facet Ping Hu
Puwen Song
Jun Xu
Qichao Wei
Ye Tao
Ye Tao
Yueming Ren
Yongang Yu
Dongxiao Li
Haiyan Hu
Chengwei Li
Chengwei Li
author_sort Ping Hu
collection DOAJ
description Serine hydroxymethyltransferase (SHMT) plays a pivotal role in cellular one-carbon, photorespiration pathways and it influences the resistance to biotic and abiotic stresses. However, the function of SHMT proteins in wheat remains largely unexplored. In the present study, SHMT genes in five Triticeae species, Oryza sativa, and four dicotyledon species were identified based on whole genome information. The origin history of the target gene was traced by micro-collinearity analysis. Gene expression patterns of TaSHMTs in different tissues, various biotic stresses, exogenous hormones, and two biotic stresses were determined by Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The function of the selected TaSHMT3A-1 was studied by barley stripe mosaic virus-induced gene silencing in common wheat Bainong207. A total of 64 SHMT members were identified and further classified into two main classes based on the structure of SHMT proteins. The gene structure and motif composition analyses revealed that SHMTs kept relatively conserved within the same subclasses. Interestingly, there was a gene, TdSHMT7B-1, on chromosome 7B of Triticum dicoccoides, but there was no SHMT gene on chromosome 7 of other analyzed Triticeae species; TdSHMT7B-1 had fewer exons and conserved motifs than the genes in the same subclass, suggesting that the gene of TdSHMT7B-1 has a notable evolutionary progress. The micro-collinearity relationship showed that no homologs of TaSHMT3A-1 and its two neighboring genes were found in the collinearity region of Triticum urartu, and there were 27 genes inserted into the collinearity region of T. urartu. Furthermore, qRT-PCR results showed that TaSHMT3A-1 was responsive to abiotic stresses (NaCl and cold), abscisic acid, methyl jasmonate, and hydrogen peroxide. Significantly, upon Fusarium graminearum infection, the expression of TaSHMT3A-1 was highly upregulated in resistant cultivar Sumai3. More importantly, silencing of TaSHMT3A-1 compromises Fusarium head blight resistance in common wheat Bainong207. Our new findings suggest that the TaSHMT3A-1 gene in wheat plays an important role in resistance to Fusarium head blight. This provides a valuable reference for further study on the function of this gene family.
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spelling doaj.art-1229c05050e44d72b11cccf1551025552022-12-21T20:09:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011310.3389/fpls.2022.847087847087Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in WheatPing Hu0Puwen Song1Jun Xu2Qichao Wei3Ye Tao4Ye Tao5Yueming Ren6Yongang Yu7Dongxiao Li8Haiyan Hu9Chengwei Li10Chengwei Li11Henan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaDepartment of Plant Protection, Sumy National Agrarian University, Sumy, UkraineHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaSerine hydroxymethyltransferase (SHMT) plays a pivotal role in cellular one-carbon, photorespiration pathways and it influences the resistance to biotic and abiotic stresses. However, the function of SHMT proteins in wheat remains largely unexplored. In the present study, SHMT genes in five Triticeae species, Oryza sativa, and four dicotyledon species were identified based on whole genome information. The origin history of the target gene was traced by micro-collinearity analysis. Gene expression patterns of TaSHMTs in different tissues, various biotic stresses, exogenous hormones, and two biotic stresses were determined by Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The function of the selected TaSHMT3A-1 was studied by barley stripe mosaic virus-induced gene silencing in common wheat Bainong207. A total of 64 SHMT members were identified and further classified into two main classes based on the structure of SHMT proteins. The gene structure and motif composition analyses revealed that SHMTs kept relatively conserved within the same subclasses. Interestingly, there was a gene, TdSHMT7B-1, on chromosome 7B of Triticum dicoccoides, but there was no SHMT gene on chromosome 7 of other analyzed Triticeae species; TdSHMT7B-1 had fewer exons and conserved motifs than the genes in the same subclass, suggesting that the gene of TdSHMT7B-1 has a notable evolutionary progress. The micro-collinearity relationship showed that no homologs of TaSHMT3A-1 and its two neighboring genes were found in the collinearity region of Triticum urartu, and there were 27 genes inserted into the collinearity region of T. urartu. Furthermore, qRT-PCR results showed that TaSHMT3A-1 was responsive to abiotic stresses (NaCl and cold), abscisic acid, methyl jasmonate, and hydrogen peroxide. Significantly, upon Fusarium graminearum infection, the expression of TaSHMT3A-1 was highly upregulated in resistant cultivar Sumai3. More importantly, silencing of TaSHMT3A-1 compromises Fusarium head blight resistance in common wheat Bainong207. Our new findings suggest that the TaSHMT3A-1 gene in wheat plays an important role in resistance to Fusarium head blight. This provides a valuable reference for further study on the function of this gene family.https://www.frontiersin.org/articles/10.3389/fpls.2022.847087/fullgenome-wide analysisSHMT gene familyexpression patternFusarium head blightvirus-induced gene silencingevolutionary progress
spellingShingle Ping Hu
Puwen Song
Jun Xu
Qichao Wei
Ye Tao
Ye Tao
Yueming Ren
Yongang Yu
Dongxiao Li
Haiyan Hu
Chengwei Li
Chengwei Li
Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
Frontiers in Plant Science
genome-wide analysis
SHMT gene family
expression pattern
Fusarium head blight
virus-induced gene silencing
evolutionary progress
title Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
title_full Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
title_fullStr Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
title_full_unstemmed Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
title_short Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat
title_sort genome wide analysis of serine hydroxymethyltransferase genes in triticeae species reveals that tashmt3a 1 regulates fusarium head blight resistance in wheat
topic genome-wide analysis
SHMT gene family
expression pattern
Fusarium head blight
virus-induced gene silencing
evolutionary progress
url https://www.frontiersin.org/articles/10.3389/fpls.2022.847087/full
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