Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i>
Thick-lipped grey mullet (<i>Chelon labrosus</i>) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of...
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MDPI AG
2020-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/5/874 |
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| author | Neda Gilannejad Verónica de las Heras Juan Antonio Martos-Sitcha Francisco J. Moyano Manuel Yúfera Gonzalo Martínez-Rodríguez |
| author_facet | Neda Gilannejad Verónica de las Heras Juan Antonio Martos-Sitcha Francisco J. Moyano Manuel Yúfera Gonzalo Martínez-Rodríguez |
| author_sort | Neda Gilannejad |
| collection | DOAJ |
| description | Thick-lipped grey mullet (<i>Chelon labrosus</i>) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (<i>try2</i>, <i>ctr</i>, <i>pga2</i>, and <i>atp4a</i>), carbohydrates (<i>amy2a</i>), and lipids (<i>cel</i> and <i>pla2g1b</i>), together with two somatotropic factors (<i>gh</i> and <i>igf1</i>) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of <i>try2</i>, <i>ctr</i>, <i>amy2a</i>, and <i>cel</i> increased continuously along development, and showed a peak at the end of metamorphosis. Expression of <i>pla2g1b</i>, <i>pga2</i> and <i>atp4a</i> increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, <i>gh</i> and <i>igf1</i>, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph. |
| first_indexed | 2024-03-10T19:45:23Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:45:23Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-122ac59f56794a3ea377be95f561e2cb2023-11-20T00:48:28ZengMDPI AGAnimals2076-26152020-05-0110587410.3390/ani10050874Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i>Neda Gilannejad0Verónica de las Heras1Juan Antonio Martos-Sitcha2Francisco J. Moyano3Manuel Yúfera4Gonzalo Martínez-Rodríguez5Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), 11519 Puerto Real, Cádiz, SpainInstituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), 11519 Puerto Real, Cádiz, SpainDepartment of Biology, Faculty of Marine and Environmental Sciences, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11519 Puerto Real, Cádiz, SpainDepartamento de Biología y Geología, Facultad de Ciencias, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Almería, La Cañada de San Urbano, 04120 Almería, SpainInstituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), 11519 Puerto Real, Cádiz, SpainInstituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), 11519 Puerto Real, Cádiz, SpainThick-lipped grey mullet (<i>Chelon labrosus</i>) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (<i>try2</i>, <i>ctr</i>, <i>pga2</i>, and <i>atp4a</i>), carbohydrates (<i>amy2a</i>), and lipids (<i>cel</i> and <i>pla2g1b</i>), together with two somatotropic factors (<i>gh</i> and <i>igf1</i>) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of <i>try2</i>, <i>ctr</i>, <i>amy2a</i>, and <i>cel</i> increased continuously along development, and showed a peak at the end of metamorphosis. Expression of <i>pla2g1b</i>, <i>pga2</i> and <i>atp4a</i> increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, <i>gh</i> and <i>igf1</i>, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph.https://www.mdpi.com/2076-2615/10/5/874<i>Chelon labrosus</i>digestive functionenzyme activitygene expressionontogenysomatotropic factors |
| spellingShingle | Neda Gilannejad Verónica de las Heras Juan Antonio Martos-Sitcha Francisco J. Moyano Manuel Yúfera Gonzalo Martínez-Rodríguez Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> Animals <i>Chelon labrosus</i> digestive function enzyme activity gene expression ontogeny somatotropic factors |
| title | Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> |
| title_full | Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> |
| title_fullStr | Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> |
| title_full_unstemmed | Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> |
| title_short | Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of <i>gh</i>/<i>igf1</i> Axis in the Omnivorous Fish <i>Chelon labrosus</i> |
| title_sort | ontogeny of expression and activity of digestive enzymes and establishment of i gh i i igf1 i axis in the omnivorous fish i chelon labrosus i |
| topic | <i>Chelon labrosus</i> digestive function enzyme activity gene expression ontogeny somatotropic factors |
| url | https://www.mdpi.com/2076-2615/10/5/874 |
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