Evaluation by hierarchical clustering of multiple cytokine expression after phytohemagglutinin stimulation
The hierarchical clustering method has been used for exploration of gene expression and proteomic profiles; however, little research into its application in the examination of expression of multiplecytokine/chemokine responses to stimuli has been reported. Thus, little progress has been mad...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600040Y.pdf |
| Summary: | The hierarchical clustering method has been used for exploration of gene
expression and proteomic profiles; however, little research into its
application in the examination of expression of multiplecytokine/chemokine
responses to stimuli has been reported. Thus, little progress has been made
on how phytohemagglutinin(PHA) affects cytokine expression profiling on a
large scale in the human hematological system. To investigate the
characteristic expression pattern under PHA stimulation, Luminex, a multiplex
bead-based suspension array, was performed. The data set collected from human
peripheral blood mononuclear cells (PBMC) was analyzed using the hierarchical
clustering method. It was revealed that two specific chemokines (CCL3
andCCL4) underwent significantly greater quantitative changes during
induction of expression than other tested cytokines/chemokines after PHA
stimulation. This result indicates that hierarchical clustering is a useful
tool for detecting fine patterns during exploration of biological data, and
that it can play an important role in comparative studies. |
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| ISSN: | 0354-4664 1821-4339 |