Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay
Shiga toxin (Stx)−producing <i>Escherichia coli</i> (STEC) and its subgroup enterohemorrhagic <i>E. coli</i> are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/9/276 |
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| author | Miriam A. Silva Anna Raquel R. Santos Leticia B. Rocha Bruna A. Caetano Thais Mitsunari Luanda I. Santos Juliana M. Polatto Denise S. P. Q. Horton Beatriz E. C. Guth Luís Fernando dos Santos Roxane M. F. Piazza |
| author_facet | Miriam A. Silva Anna Raquel R. Santos Leticia B. Rocha Bruna A. Caetano Thais Mitsunari Luanda I. Santos Juliana M. Polatto Denise S. P. Q. Horton Beatriz E. C. Guth Luís Fernando dos Santos Roxane M. F. Piazza |
| author_sort | Miriam A. Silva |
| collection | DOAJ |
| description | Shiga toxin (Stx)−producing <i>Escherichia coli</i> (STEC) and its subgroup enterohemorrhagic <i>E. coli</i> are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx<sub>1</sub> and Stx<sub>2</sub> toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx<sub>1</sub> plus mAb stx<sub>2</sub>) showed 99% sensitivity and 97% specificity. Individually, Stx<sub>1</sub> antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx<sub>2</sub> antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections. |
| first_indexed | 2024-12-21T12:53:39Z |
| format | Article |
| id | doaj.art-1230c81a3df8435093ec805fdb058b5e |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-21T12:53:39Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1230c81a3df8435093ec805fdb058b5e2022-12-21T19:03:25ZengMDPI AGMicroorganisms2076-26072019-08-017927610.3390/microorganisms7090276microorganisms7090276Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic AssayMiriam A. Silva0Anna Raquel R. Santos1Leticia B. Rocha2Bruna A. Caetano3Thais Mitsunari4Luanda I. Santos5Juliana M. Polatto6Denise S. P. Q. Horton7Beatriz E. C. Guth8Luís Fernando dos Santos9Roxane M. F. Piazza10Laboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilDepartamento de Microbiologia, Imunologia, Parasitologia, Escola Paulista de Medicina Universidade Federal de São Paulo, São Paulo 04023-062, BrasilCentro de Bacteriologia, Instituto Adolfo Lutz, São Paulo 01246-000, BrasilLaboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, BrasilShiga toxin (Stx)−producing <i>Escherichia coli</i> (STEC) and its subgroup enterohemorrhagic <i>E. coli</i> are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx<sub>1</sub> and Stx<sub>2</sub> toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx<sub>1</sub> plus mAb stx<sub>2</sub>) showed 99% sensitivity and 97% specificity. Individually, Stx<sub>1</sub> antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx<sub>2</sub> antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections.https://www.mdpi.com/2076-2607/7/9/276STECShiga toxinsantibodiescapture ELISAlatex agglutination testlateral flow assay |
| spellingShingle | Miriam A. Silva Anna Raquel R. Santos Leticia B. Rocha Bruna A. Caetano Thais Mitsunari Luanda I. Santos Juliana M. Polatto Denise S. P. Q. Horton Beatriz E. C. Guth Luís Fernando dos Santos Roxane M. F. Piazza Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay Microorganisms STEC Shiga toxins antibodies capture ELISA latex agglutination test lateral flow assay |
| title | Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay |
| title_full | Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay |
| title_fullStr | Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay |
| title_full_unstemmed | Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay |
| title_short | Development and Validation of Shiga Toxin-Producing <i>Escherichia coli</i> Immunodiagnostic Assay |
| title_sort | development and validation of shiga toxin producing i escherichia coli i immunodiagnostic assay |
| topic | STEC Shiga toxins antibodies capture ELISA latex agglutination test lateral flow assay |
| url | https://www.mdpi.com/2076-2607/7/9/276 |
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