The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates
Comparative genomics has greatly facilitated the identification of shared as well as unique features among individual cells or tissues, and thus offers the potential to find disease markers. While proteomics is recognized for its potential to generate quantitative maps of protein expression, compara...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/10/1129 |
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| author | Jelena Erdmann Janne G. Thöming Sarah Pohl Andreas Pich Christof Lenz Susanne Häussler |
| author_facet | Jelena Erdmann Janne G. Thöming Sarah Pohl Andreas Pich Christof Lenz Susanne Häussler |
| author_sort | Jelena Erdmann |
| collection | DOAJ |
| description | Comparative genomics has greatly facilitated the identification of shared as well as unique features among individual cells or tissues, and thus offers the potential to find disease markers. While proteomics is recognized for its potential to generate quantitative maps of protein expression, comparative proteomics in bacteria has been largely restricted to the comparison of single cell lines or mutant strains. In this study, we used a data independent acquisition (DIA) technique, which enables global protein quantification of large sample cohorts, to record the proteome profiles of overall 27 whole genome sequenced and transcriptionally profiled clinical isolates of the opportunistic pathogen <i>Pseudomonas aeruginosa.</i> Analysis of the proteome profiles across the 27 clinical isolates grown under planktonic and biofilm growth conditions led to the identification of a core biofilm-associated protein profile. Furthermore, we found that protein-to-mRNA ratios between different <i>P. aeruginosa</i> strains are well correlated, indicating conserved patterns of post-transcriptional regulation. Uncovering core regulatory pathways, which drive biofilm formation and associated antibiotic tolerance in bacterial pathogens, promise to give clues to interactions between bacterial species and their environment and could provide useful targets for new clinical interventions to combat biofilm-associated infections. |
| first_indexed | 2024-03-12T18:35:35Z |
| format | Article |
| id | doaj.art-123513b5baee448e9fdf2b593331be3b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T18:35:35Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-123513b5baee448e9fdf2b593331be3b2023-08-02T08:00:44ZengMDPI AGCells2073-44092019-09-01810112910.3390/cells8101129cells8101129The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> IsolatesJelena Erdmann0Janne G. Thöming1Sarah Pohl2Andreas Pich3Christof Lenz4Susanne Häussler5Institute for Molecular Bacteriology, TWINCORE GmbH, Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover 30625, GermanyInstitute for Molecular Bacteriology, TWINCORE GmbH, Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover 30625, GermanyInstitute for Molecular Bacteriology, TWINCORE GmbH, Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover 30625, GermanyResearch Core Unit Proteomics and Institute of Toxicology, Hannover Medical School, Hannover 30625, GermanyInstitute of Clinical Chemistry, Bioanalytics, University Medical Center Göttingen, Göttingen 37075, GermanyInstitute for Molecular Bacteriology, TWINCORE GmbH, Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover 30625, GermanyComparative genomics has greatly facilitated the identification of shared as well as unique features among individual cells or tissues, and thus offers the potential to find disease markers. While proteomics is recognized for its potential to generate quantitative maps of protein expression, comparative proteomics in bacteria has been largely restricted to the comparison of single cell lines or mutant strains. In this study, we used a data independent acquisition (DIA) technique, which enables global protein quantification of large sample cohorts, to record the proteome profiles of overall 27 whole genome sequenced and transcriptionally profiled clinical isolates of the opportunistic pathogen <i>Pseudomonas aeruginosa.</i> Analysis of the proteome profiles across the 27 clinical isolates grown under planktonic and biofilm growth conditions led to the identification of a core biofilm-associated protein profile. Furthermore, we found that protein-to-mRNA ratios between different <i>P. aeruginosa</i> strains are well correlated, indicating conserved patterns of post-transcriptional regulation. Uncovering core regulatory pathways, which drive biofilm formation and associated antibiotic tolerance in bacterial pathogens, promise to give clues to interactions between bacterial species and their environment and could provide useful targets for new clinical interventions to combat biofilm-associated infections.https://www.mdpi.com/2073-4409/8/10/1129bacteriaDIAmass spectrometrymicrobiologySWATH |
| spellingShingle | Jelena Erdmann Janne G. Thöming Sarah Pohl Andreas Pich Christof Lenz Susanne Häussler The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates Cells bacteria DIA mass spectrometry microbiology SWATH |
| title | The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates |
| title_full | The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates |
| title_fullStr | The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates |
| title_full_unstemmed | The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates |
| title_short | The Core Proteome of Biofilm-Grown Clinical <i>Pseudomonas aeruginosa</i> Isolates |
| title_sort | core proteome of biofilm grown clinical i pseudomonas aeruginosa i isolates |
| topic | bacteria DIA mass spectrometry microbiology SWATH |
| url | https://www.mdpi.com/2073-4409/8/10/1129 |
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