Exploring Metal Ion Coordination to Nucleic Acids by NMR
Metal ions play a crucial role in charge compensation, folding and stabilization of tertiary structures of large nucleic acids. In addition, they may be directly involved in the catalytic mechanism of ribozymes. Most metal ions applied in the context of nucleic acids in vivo and in vitro b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2009-03-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/4632 |
| _version_ | 1819278654444666880 |
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| author | Silke Johannsen Maximiliane M. T. Korth Joachim Schnabl Roland K. O. Sigel |
| author_facet | Silke Johannsen Maximiliane M. T. Korth Joachim Schnabl Roland K. O. Sigel |
| author_sort | Silke Johannsen |
| collection | DOAJ |
| description |
Metal ions play a crucial role in charge compensation, folding and stabilization of tertiary structures of large nucleic acids. In addition, they may be directly involved in the catalytic mechanism of ribozymes. Most metal ions applied in the context of nucleic acids in vivo
and in vitro bind in a kinetically labile fashion. Hence, the detection of metal ion binding sites, not to mention the elucidation of the specific coordination sphere, still poses largely unresolved problems. Here we describe the different strategies applied and the progress made over
the last years to characterize metal ion coordination to large nucleic acids by NMR.
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| first_indexed | 2024-12-24T00:15:27Z |
| format | Article |
| id | doaj.art-1235a069f2a849b198ebee0b637bae9a |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-24T00:15:27Z |
| publishDate | 2009-03-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-1235a069f2a849b198ebee0b637bae9a2022-12-21T17:24:44ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242009-03-0163310.2533/chimia.2009.146Exploring Metal Ion Coordination to Nucleic Acids by NMRSilke JohannsenMaximiliane M. T. KorthJoachim SchnablRoland K. O. Sigel Metal ions play a crucial role in charge compensation, folding and stabilization of tertiary structures of large nucleic acids. In addition, they may be directly involved in the catalytic mechanism of ribozymes. Most metal ions applied in the context of nucleic acids in vivo and in vitro bind in a kinetically labile fashion. Hence, the detection of metal ion binding sites, not to mention the elucidation of the specific coordination sphere, still poses largely unresolved problems. Here we describe the different strategies applied and the progress made over the last years to characterize metal ion coordination to large nucleic acids by NMR. https://www.chimia.ch/chimia/article/view/4632DnaMetal ionsNmrRibozymesRna |
| spellingShingle | Silke Johannsen Maximiliane M. T. Korth Joachim Schnabl Roland K. O. Sigel Exploring Metal Ion Coordination to Nucleic Acids by NMR CHIMIA Dna Metal ions Nmr Ribozymes Rna |
| title | Exploring Metal Ion Coordination to Nucleic Acids by NMR |
| title_full | Exploring Metal Ion Coordination to Nucleic Acids by NMR |
| title_fullStr | Exploring Metal Ion Coordination to Nucleic Acids by NMR |
| title_full_unstemmed | Exploring Metal Ion Coordination to Nucleic Acids by NMR |
| title_short | Exploring Metal Ion Coordination to Nucleic Acids by NMR |
| title_sort | exploring metal ion coordination to nucleic acids by nmr |
| topic | Dna Metal ions Nmr Ribozymes Rna |
| url | https://www.chimia.ch/chimia/article/view/4632 |
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