Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China
Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/8/517 |
| _version_ | 1797583049354903552 |
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| author | Menghang Wang Ying Yu Jianan Wu Shujie Wang Luis G Giménez-Lirola Pablo Piñeyro Yu Wang Hongliang Cui Xijun He Jeffrey J. Zimmerman Yabin Tu Xuehui Cai Gang Wang |
| author_facet | Menghang Wang Ying Yu Jianan Wu Shujie Wang Luis G Giménez-Lirola Pablo Piñeyro Yu Wang Hongliang Cui Xijun He Jeffrey J. Zimmerman Yabin Tu Xuehui Cai Gang Wang |
| author_sort | Menghang Wang |
| collection | DOAJ |
| description | Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding the pathogenicity of PCV3, mostly due to the limited number of PCV3 isolates that are readily available. In this study, PCV3-DB-1 was isolated in PK-15 cells and characterized in vitro. Electron microscopy revealed the presence of PCV-like particles, and in situ <i>hybridization</i> RNA analysis demonstrated the replication of PCV3 in PK-15 cell culture. Based on phylogenetic analysis of PCV3 isolates from the Heilongjiang province of China, PCV3-DB-1 with 24 alanine and 27 lysine in the Cap protein was originally isolated and determined to belong to the clade PCV3a. |
| first_indexed | 2024-03-10T23:30:21Z |
| format | Article |
| id | doaj.art-123634dc7c434221b62b2d0fc10b33b4 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T23:30:21Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-123634dc7c434221b62b2d0fc10b33b42023-11-19T03:19:04ZengMDPI AGVeterinary Sciences2306-73812023-08-0110851710.3390/vetsci10080517Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast ChinaMenghang Wang0Ying Yu1Jianan Wu2Shujie Wang3Luis G Giménez-Lirola4Pablo Piñeyro5Yu Wang6Hongliang Cui7Xijun He8Jeffrey J. Zimmerman9Yabin Tu10Xuehui Cai11Gang Wang12Heilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaHeilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaPorcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding the pathogenicity of PCV3, mostly due to the limited number of PCV3 isolates that are readily available. In this study, PCV3-DB-1 was isolated in PK-15 cells and characterized in vitro. Electron microscopy revealed the presence of PCV-like particles, and in situ <i>hybridization</i> RNA analysis demonstrated the replication of PCV3 in PK-15 cell culture. Based on phylogenetic analysis of PCV3 isolates from the Heilongjiang province of China, PCV3-DB-1 with 24 alanine and 27 lysine in the Cap protein was originally isolated and determined to belong to the clade PCV3a.https://www.mdpi.com/2306-7381/10/8/517porcine circovirusPCV3 isolationPCV-like particlesgenomic characteristics |
| spellingShingle | Menghang Wang Ying Yu Jianan Wu Shujie Wang Luis G Giménez-Lirola Pablo Piñeyro Yu Wang Hongliang Cui Xijun He Jeffrey J. Zimmerman Yabin Tu Xuehui Cai Gang Wang Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China Veterinary Sciences porcine circovirus PCV3 isolation PCV-like particles genomic characteristics |
| title | Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China |
| title_full | Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China |
| title_fullStr | Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China |
| title_full_unstemmed | Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China |
| title_short | Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China |
| title_sort | genetic and in vitro characteristics of a porcine circovirus type 3 isolate from northeast china |
| topic | porcine circovirus PCV3 isolation PCV-like particles genomic characteristics |
| url | https://www.mdpi.com/2306-7381/10/8/517 |
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