The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles
Abiotic stress caused by drought impairs plant growth and reduces yields. This study aimed to investigate the impact of silica nanoparticles (SiO<sub>2</sub> NPs) through the adverse effects of drought on the growth, oxidative stress, and antioxidative response of pea ‘Respect’. Pea plan...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/1/35 |
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| author | Rūta Sutulienė Lina Ragelienė Giedrė Samuolienė Aušra Brazaitytė Martynas Urbutis Jurga Miliauskienė |
| author_facet | Rūta Sutulienė Lina Ragelienė Giedrė Samuolienė Aušra Brazaitytė Martynas Urbutis Jurga Miliauskienė |
| author_sort | Rūta Sutulienė |
| collection | DOAJ |
| description | Abiotic stress caused by drought impairs plant growth and reduces yields. This study aimed to investigate the impact of silica nanoparticles (SiO<sub>2</sub> NPs) through the adverse effects of drought on the growth, oxidative stress, and antioxidative response of pea ‘Respect’. Pea plants were grown in a greenhouse before being watered (100 ± 1 mL per pot) or foliar sprayed (ca. 14 ± 0.5 mL plant<sup>−1</sup>) with suspensions containing SiO<sub>2</sub> NPs (0, 12.5 ppm, 25 ppm, and 50 ppm) and were exposed to drought stress for 10 days. Drought stress was created by maintaining 30% of the soil moisture while the control was 80%. The growth parameters of pea grown under drought stress conditions were improved by spraying or watering plants with SiO<sub>2</sub> NPs (12.5, 25, and 50 ppm). At drought stress, peas treated with SiO<sub>2</sub> NPs (50 ppm) increased their relative water content by 29%, specific leaf area by 17%, and decreased root/shoot ratio by 4% as compared to plant non-treated with SiO<sub>2</sub> NPs. In addition, spraying or watering of SiO<sub>2</sub> NPs increased peas tolerance to drought by increasing the activity of antioxidant enzymes at least three times including catalase, ascorbate peroxidase, glutathione reductase, and superoxide dismutase, as well as reducing hydrogen peroxide and lipid peroxidation in plant tissue. It was observed the increase in total phenolic compounds and non-enzymatic antioxidant activity (DPPH, ABTS, FRAP) in peas treated with SiO<sub>2</sub> NPs under drought stress. The physiological response of peas to drought and the effects of SiO<sub>2</sub> NPs studied in this experiment based on the use of the concentration of 50 ppm nanoparticles can protect peas from the damaging effects of drought and could help reduce global food shortages. |
| first_indexed | 2024-03-10T01:21:36Z |
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| id | doaj.art-12371158cd5242a0bab5e32317f36b17 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T01:21:36Z |
| publishDate | 2021-12-01 |
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| series | Horticulturae |
| spelling | doaj.art-12371158cd5242a0bab5e32317f36b172023-11-23T13:57:34ZengMDPI AGHorticulturae2311-75242021-12-01813510.3390/horticulturae8010035The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica NanoparticlesRūta Sutulienė0Lina Ragelienė1Giedrė Samuolienė2Aušra Brazaitytė3Martynas Urbutis4Jurga Miliauskienė5Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, LT-54333 Babtai, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT-44404 Kaunas, LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, LT-54333 Babtai, LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, LT-54333 Babtai, LithuaniaUAB Ikarai, Vakarų Str. 6, LT-57238 Kėdainiai, LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas Str. 30, LT-54333 Babtai, LithuaniaAbiotic stress caused by drought impairs plant growth and reduces yields. This study aimed to investigate the impact of silica nanoparticles (SiO<sub>2</sub> NPs) through the adverse effects of drought on the growth, oxidative stress, and antioxidative response of pea ‘Respect’. Pea plants were grown in a greenhouse before being watered (100 ± 1 mL per pot) or foliar sprayed (ca. 14 ± 0.5 mL plant<sup>−1</sup>) with suspensions containing SiO<sub>2</sub> NPs (0, 12.5 ppm, 25 ppm, and 50 ppm) and were exposed to drought stress for 10 days. Drought stress was created by maintaining 30% of the soil moisture while the control was 80%. The growth parameters of pea grown under drought stress conditions were improved by spraying or watering plants with SiO<sub>2</sub> NPs (12.5, 25, and 50 ppm). At drought stress, peas treated with SiO<sub>2</sub> NPs (50 ppm) increased their relative water content by 29%, specific leaf area by 17%, and decreased root/shoot ratio by 4% as compared to plant non-treated with SiO<sub>2</sub> NPs. In addition, spraying or watering of SiO<sub>2</sub> NPs increased peas tolerance to drought by increasing the activity of antioxidant enzymes at least three times including catalase, ascorbate peroxidase, glutathione reductase, and superoxide dismutase, as well as reducing hydrogen peroxide and lipid peroxidation in plant tissue. It was observed the increase in total phenolic compounds and non-enzymatic antioxidant activity (DPPH, ABTS, FRAP) in peas treated with SiO<sub>2</sub> NPs under drought stress. The physiological response of peas to drought and the effects of SiO<sub>2</sub> NPs studied in this experiment based on the use of the concentration of 50 ppm nanoparticles can protect peas from the damaging effects of drought and could help reduce global food shortages.https://www.mdpi.com/2311-7524/8/1/35antioxidant activitydroughtgreen peaoxidative stress biomarkersSiO<sub>2</sub> nanoparticles |
| spellingShingle | Rūta Sutulienė Lina Ragelienė Giedrė Samuolienė Aušra Brazaitytė Martynas Urbutis Jurga Miliauskienė The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles Horticulturae antioxidant activity drought green pea oxidative stress biomarkers SiO<sub>2</sub> nanoparticles |
| title | The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles |
| title_full | The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles |
| title_fullStr | The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles |
| title_full_unstemmed | The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles |
| title_short | The Response of Antioxidant System of Drought-Stressed Green Pea (<i>Pisum sativum</i> L.) Affected by Watering and Foliar Spray with Silica Nanoparticles |
| title_sort | response of antioxidant system of drought stressed green pea i pisum sativum i l affected by watering and foliar spray with silica nanoparticles |
| topic | antioxidant activity drought green pea oxidative stress biomarkers SiO<sub>2</sub> nanoparticles |
| url | https://www.mdpi.com/2311-7524/8/1/35 |
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