Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber
Gummy stem blight (GSB), caused by Didymella bryoniae, is one of the most devastating diseases that severely reduces cucumber production. Developing resistant varieties would be an effective strategy to control GSB. Although several GSB-resistant QTLs have been reported, causal genes for GSB resista...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.968811/full |
| _version_ | 1811277667659415552 |
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| author | Jianan Han Shaoyun Dong Xiaoping Liu Yanxia Shi Diane M. Beckles Xingfang Gu Han Miao Shengping Zhang |
| author_facet | Jianan Han Shaoyun Dong Xiaoping Liu Yanxia Shi Diane M. Beckles Xingfang Gu Han Miao Shengping Zhang |
| author_sort | Jianan Han |
| collection | DOAJ |
| description | Gummy stem blight (GSB), caused by Didymella bryoniae, is one of the most devastating diseases that severely reduces cucumber production. Developing resistant varieties would be an effective strategy to control GSB. Although several GSB-resistant QTLs have been reported, causal genes for GSB resistance have not yet been identified in cucumber. A novel loci gsb3.1 for seedling GSB resistance from the “PI 183967” genotype was previously identified in a 1.7-Mb interval on chromosome 3. In this study, we developed a residual heterozygous line-derived strategy from Recombinant Inbred Lines to perform fine mapping, and with this approach, the gsb3.1 locus was narrowed to a 38 kb interval. There were six predicted genes at the gsb3.1 locus, four of which differed in expression in the GSB-resistant compared to the susceptible lines after fungal inoculation. These candidate genes (Csa3G020050, Csa3G020060, Csa3G020090, and Csa3G020590) within the gsb3.1 locus could be helpful for the genetic study of GSB resistance and marker-assisted selection in cucumber. Phylogenetic analyses indicated that the resistant gsb3.1 allele may uniquely exist in the wild species present in the Indian group, and that nucleotide diversity was significantly reduced in cultivated accessions. Therefore, the gsb3.1 allele could be introgressed into existing commercial cultivars and combined with other resistance QTLs to provide broad-spectrum and robust GSB resistance in cucumber. |
| first_indexed | 2024-04-13T00:20:53Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-04-13T00:20:53Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-1238a07139e94b928b728ad1fd1ca7142022-12-22T03:10:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.968811968811Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumberJianan Han0Shaoyun Dong1Xiaoping Liu2Yanxia Shi3Diane M. Beckles4Xingfang Gu5Han Miao6Shengping Zhang7Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Sciences, University of California, Davis, Davis, CA, United StatesInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaGummy stem blight (GSB), caused by Didymella bryoniae, is one of the most devastating diseases that severely reduces cucumber production. Developing resistant varieties would be an effective strategy to control GSB. Although several GSB-resistant QTLs have been reported, causal genes for GSB resistance have not yet been identified in cucumber. A novel loci gsb3.1 for seedling GSB resistance from the “PI 183967” genotype was previously identified in a 1.7-Mb interval on chromosome 3. In this study, we developed a residual heterozygous line-derived strategy from Recombinant Inbred Lines to perform fine mapping, and with this approach, the gsb3.1 locus was narrowed to a 38 kb interval. There were six predicted genes at the gsb3.1 locus, four of which differed in expression in the GSB-resistant compared to the susceptible lines after fungal inoculation. These candidate genes (Csa3G020050, Csa3G020060, Csa3G020090, and Csa3G020590) within the gsb3.1 locus could be helpful for the genetic study of GSB resistance and marker-assisted selection in cucumber. Phylogenetic analyses indicated that the resistant gsb3.1 allele may uniquely exist in the wild species present in the Indian group, and that nucleotide diversity was significantly reduced in cultivated accessions. Therefore, the gsb3.1 allele could be introgressed into existing commercial cultivars and combined with other resistance QTLs to provide broad-spectrum and robust GSB resistance in cucumber.https://www.frontiersin.org/articles/10.3389/fpls.2022.968811/fullCucumis sativus L.gummy stem blightresidual heterozygous line (RHL)-derived strategyfine-mappingcandidate genes |
| spellingShingle | Jianan Han Shaoyun Dong Xiaoping Liu Yanxia Shi Diane M. Beckles Xingfang Gu Han Miao Shengping Zhang Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber Frontiers in Plant Science Cucumis sativus L. gummy stem blight residual heterozygous line (RHL)-derived strategy fine-mapping candidate genes |
| title | Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber |
| title_full | Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber |
| title_fullStr | Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber |
| title_full_unstemmed | Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber |
| title_short | Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber |
| title_sort | fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines derived strategy in cucumber |
| topic | Cucumis sativus L. gummy stem blight residual heterozygous line (RHL)-derived strategy fine-mapping candidate genes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.968811/full |
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