The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma
Abstract Background TP53 is the most frequently mutated gene in human cancers. Previous studies reported that TP53 mutations correlated with poor prognoses in patients with head and neck squamous cell carcinoma (HNSCC). However, the relationship between TP53 mutations and hypopharyngeal squamous cel...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-017-3913-1 |
| _version_ | 1811259141945032704 |
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| author | Go Omura Mizuo Ando Yasuhiro Ebihara Yuki Saito Kenya Kobayashi Osamu Fukuoka Ken Akashi Masafumi Yoshida Takahiro Asakage Tatsuya Yamasoba |
| author_facet | Go Omura Mizuo Ando Yasuhiro Ebihara Yuki Saito Kenya Kobayashi Osamu Fukuoka Ken Akashi Masafumi Yoshida Takahiro Asakage Tatsuya Yamasoba |
| author_sort | Go Omura |
| collection | DOAJ |
| description | Abstract Background TP53 is the most frequently mutated gene in human cancers. Previous studies reported that TP53 mutations correlated with poor prognoses in patients with head and neck squamous cell carcinoma (HNSCC). However, the relationship between TP53 mutations and hypopharyngeal squamous cell carcinoma (HPSCC) is not known. The current study aimed to evaluate TP53 mutation status as a predictive biomarker in patients with HPSCC. Methods We retrospectively reviewed the clinical charts of 57 HPSCC patients treated with initial surgery between 2008 and 2014. TP53 mutation status was determined by Sanger sequencing, and patients were classified into wild-type, missense mutation, and truncating mutation groups. Additionally, p53 expression was determined using immunohistochemistry in surgical specimens. Results TP53 mutations were identified in 39 (68%) patients. The 3-year disease-specific survival (DSS) rate of wild-type, missense mutation, and truncating mutation group were 94%, 61%, and 43%, respectively. The TP53 mutation group displayed significantly worse DSS and overall survival rates than the wild-type group (P = 0.01 and P = 0.007, respectively). Multivariate analyses revealed that the presence of TP53 mutations and ≥4 metastatic lymph nodes were independent adverse prognostic factors for HPSCC. p53 immunopositivity was detected in 22 patients, including 5 (28%) and 17 (71%) patients in the wild-type and missense mutation groups, whereas none of the patients with truncating mutation exhibited p53 immunopositivity (P = 0.0001). Conclusion The TP53 mutation status correlated with poor prognosis in surgically treated HPSCC patients. Specifically, truncating mutations which were not detected by p53 immunohistochemistry were predictive of worst survival. |
| first_indexed | 2024-04-12T18:27:00Z |
| format | Article |
| id | doaj.art-1238d96f671a4ffcb48997820f085a0d |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-12T18:27:00Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-1238d96f671a4ffcb48997820f085a0d2022-12-22T03:21:13ZengBMCBMC Cancer1471-24072017-12-0117111010.1186/s12885-017-3913-1The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinomaGo Omura0Mizuo Ando1Yasuhiro Ebihara2Yuki Saito3Kenya Kobayashi4Osamu Fukuoka5Ken Akashi6Masafumi Yoshida7Takahiro Asakage8Tatsuya Yamasoba9Department of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoDepartment of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, The University of TokyoAbstract Background TP53 is the most frequently mutated gene in human cancers. Previous studies reported that TP53 mutations correlated with poor prognoses in patients with head and neck squamous cell carcinoma (HNSCC). However, the relationship between TP53 mutations and hypopharyngeal squamous cell carcinoma (HPSCC) is not known. The current study aimed to evaluate TP53 mutation status as a predictive biomarker in patients with HPSCC. Methods We retrospectively reviewed the clinical charts of 57 HPSCC patients treated with initial surgery between 2008 and 2014. TP53 mutation status was determined by Sanger sequencing, and patients were classified into wild-type, missense mutation, and truncating mutation groups. Additionally, p53 expression was determined using immunohistochemistry in surgical specimens. Results TP53 mutations were identified in 39 (68%) patients. The 3-year disease-specific survival (DSS) rate of wild-type, missense mutation, and truncating mutation group were 94%, 61%, and 43%, respectively. The TP53 mutation group displayed significantly worse DSS and overall survival rates than the wild-type group (P = 0.01 and P = 0.007, respectively). Multivariate analyses revealed that the presence of TP53 mutations and ≥4 metastatic lymph nodes were independent adverse prognostic factors for HPSCC. p53 immunopositivity was detected in 22 patients, including 5 (28%) and 17 (71%) patients in the wild-type and missense mutation groups, whereas none of the patients with truncating mutation exhibited p53 immunopositivity (P = 0.0001). Conclusion The TP53 mutation status correlated with poor prognosis in surgically treated HPSCC patients. Specifically, truncating mutations which were not detected by p53 immunohistochemistry were predictive of worst survival.http://link.springer.com/article/10.1186/s12885-017-3913-1TP53 mutationHypopharyngeal squamous cell carcinomaTruncating mutationPrognosisPharyngectomy |
| spellingShingle | Go Omura Mizuo Ando Yasuhiro Ebihara Yuki Saito Kenya Kobayashi Osamu Fukuoka Ken Akashi Masafumi Yoshida Takahiro Asakage Tatsuya Yamasoba The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma BMC Cancer TP53 mutation Hypopharyngeal squamous cell carcinoma Truncating mutation Prognosis Pharyngectomy |
| title | The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma |
| title_full | The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma |
| title_fullStr | The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma |
| title_full_unstemmed | The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma |
| title_short | The prognostic value of TP53 mutations in hypopharyngeal squamous cell carcinoma |
| title_sort | prognostic value of tp53 mutations in hypopharyngeal squamous cell carcinoma |
| topic | TP53 mutation Hypopharyngeal squamous cell carcinoma Truncating mutation Prognosis Pharyngectomy |
| url | http://link.springer.com/article/10.1186/s12885-017-3913-1 |
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