eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex
Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa)-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-11-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124713005536 |
| _version_ | 1818234330810417152 |
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| author | José Manuel Mingot Sonia Vega Amparo Cano Francisco Portillo M. Angela Nieto |
| author_facet | José Manuel Mingot Sonia Vega Amparo Cano Francisco Portillo M. Angela Nieto |
| author_sort | José Manuel Mingot |
| collection | DOAJ |
| description | Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa)-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors. |
| first_indexed | 2024-12-12T11:36:22Z |
| format | Article |
| id | doaj.art-1238dbdbf7c546c7876ac994a11e84dc |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T11:36:22Z |
| publishDate | 2013-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-1238dbdbf7c546c7876ac994a11e84dc2022-12-22T00:25:38ZengElsevierCell Reports2211-12472013-11-015372773710.1016/j.celrep.2013.09.030eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA ComplexJosé Manuel Mingot0Sonia Vega1Amparo Cano2Francisco Portillo3M. Angela Nieto4Instituto de Neurociencias, CSIC-UMH, Avda. Ramón y Cajal s/n, 03550 San Juan de Alicante, SpainInstituto de Neurociencias, CSIC-UMH, Avda. Ramón y Cajal s/n, 03550 San Juan de Alicante, SpainDepartamento de Bioquímica UAM-Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, IdiPAZ, Arturo Duperier 4, 28029 Madrid, SpainDepartamento de Bioquímica UAM-Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, IdiPAZ, Arturo Duperier 4, 28029 Madrid, SpainInstituto de Neurociencias, CSIC-UMH, Avda. Ramón y Cajal s/n, 03550 San Juan de Alicante, SpainExportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa)-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors.http://www.sciencedirect.com/science/article/pii/S2211124713005536 |
| spellingShingle | José Manuel Mingot Sonia Vega Amparo Cano Francisco Portillo M. Angela Nieto eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex Cell Reports |
| title | eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex |
| title_full | eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex |
| title_fullStr | eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex |
| title_full_unstemmed | eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex |
| title_short | eEF1A Mediates the Nuclear Export of SNAG-Containing Proteins via the Exportin5-Aminoacyl-tRNA Complex |
| title_sort | eef1a mediates the nuclear export of snag containing proteins via the exportin5 aminoacyl trna complex |
| url | http://www.sciencedirect.com/science/article/pii/S2211124713005536 |
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