Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study
An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open q...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/9/2481 |
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| author | Vito Cela Simona Daniele Maria Elena Rosa Obino Maria Ruggiero Elisa Zappelli Lorenzo Ceccarelli Francesca Papini Ilaria Marzi Giorgia Scarfò Fulvia Tosi Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini |
| author_facet | Vito Cela Simona Daniele Maria Elena Rosa Obino Maria Ruggiero Elisa Zappelli Lorenzo Ceccarelli Francesca Papini Ilaria Marzi Giorgia Scarfò Fulvia Tosi Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini |
| author_sort | Vito Cela |
| collection | DOAJ |
| description | An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF. |
| first_indexed | 2024-03-10T04:01:05Z |
| format | Article |
| id | doaj.art-12394c61811c463db4e708ed48a7ae74 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T04:01:05Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-12394c61811c463db4e708ed48a7ae742023-11-23T08:32:56ZengMDPI AGJournal of Clinical Medicine2077-03832022-04-01119248110.3390/jcm11092481Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot StudyVito Cela0Simona Daniele1Maria Elena Rosa Obino2Maria Ruggiero3Elisa Zappelli4Lorenzo Ceccarelli5Francesca Papini6Ilaria Marzi7Giorgia Scarfò8Fulvia Tosi9Ferdinando Franzoni10Claudia Martini11Paolo Giovanni Artini12Division of Gynecology and Obstetrics, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalyDepartment of Pharmacy, University of Pisa, 56100 Pisa, ItalyDivision of Gynecology and Obstetrics, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalySan Rossore Clinic Care, 56100 Pisa, ItalyDepartment of Pharmacy, University of Pisa, 56100 Pisa, ItalyDepartment of Pharmacy, University of Pisa, 56100 Pisa, ItalyDivision of Gynecology and Obstetrics, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalyDivision of Gynecology and Obstetrics, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalyDivision of General Medicine, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalySan Rossore Clinic Care, 56100 Pisa, ItalyDivision of General Medicine, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalyDepartment of Pharmacy, University of Pisa, 56100 Pisa, ItalyDivision of Gynecology and Obstetrics, Department of Clinical and Experimental Medicine, University of Pisa, 56100 Pisa, ItalyAn abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading to repeated implantation failure (RIF) in women undergoing in vitro fertilization (IVF). However, the molecular mechanisms linking uterine microbiota and IVF out-comes are still an open question. The aim of this cohort study was to outline the relationship between endometrial microbiota, inflammation and IVF outcomes. To this purpose, endometrial microbiota and selected components of the “cytokine network” were analyzed in women presenting RIF and divided between eubiosis and dysbiosis groups, according to the percentage of endometrial lactobacilli (≥90% or <90%, respectively). The Dysbiosis group presented significantly higher tissue concentrations of the inflammatory markers (IL-6, IL-1β, HIF-1α and COX-2) and significantly lower levels of the anti-inflammatory/well-being factors, IL-10 and IGF-1, with respect to women with eubiosis. Moreover, the Lactobacillus percentage was negatively related to the concentrations of the inflammatory molecules and positively related to IL-10/IGF-1. Interestingly, the number of IVF attempts was directly related to the levels of the inflammatory factors COX-2, IL-1β and HIF-1α in the eubiosis group. Overall, endometrial dysbiosis was demonstrated to be associated with inflammation-related endometrial changes affecting the process of embryo implantation, underlining the importance of assessing uterine microbiota in patients undergoing IVF.https://www.mdpi.com/2077-0383/11/9/2481endometrial microbiotadysbiosisinflammationin vitro fertilizationrepeated implantation failure |
| spellingShingle | Vito Cela Simona Daniele Maria Elena Rosa Obino Maria Ruggiero Elisa Zappelli Lorenzo Ceccarelli Francesca Papini Ilaria Marzi Giorgia Scarfò Fulvia Tosi Ferdinando Franzoni Claudia Martini Paolo Giovanni Artini Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study Journal of Clinical Medicine endometrial microbiota dysbiosis inflammation in vitro fertilization repeated implantation failure |
| title | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
| title_full | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
| title_fullStr | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
| title_full_unstemmed | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
| title_short | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study |
| title_sort | endometrial dysbiosis is related to inflammatory factors in women with repeated implantation failure a pilot study |
| topic | endometrial microbiota dysbiosis inflammation in vitro fertilization repeated implantation failure |
| url | https://www.mdpi.com/2077-0383/11/9/2481 |
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