Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation
Lung injuries are attributed due to exposure to Drugs or chemicals. One of the important challenging situations for the clinicians is to manage treatments of different diseases with acute lung injury (ALI). The objective of this study was to investigate the possible protective mechanisms and action...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Saudi Journal of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X22000997 |
| _version_ | 1798022693775212544 |
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| author | Naif O. Al-Harbi Faisal Imam Mohammad Matar Al-Harbi Khaldoon Aljeryan Othman A. Al-Shabanah Khaled A. Alhosaini Lamya Saif Alqahtani Muhammad Afzal M.D. Khalid Anwer Abdullah A. Aldossari Mohammed M. Alanazi Sary Alsanea Mohammed A. Assiri |
| author_facet | Naif O. Al-Harbi Faisal Imam Mohammad Matar Al-Harbi Khaldoon Aljeryan Othman A. Al-Shabanah Khaled A. Alhosaini Lamya Saif Alqahtani Muhammad Afzal M.D. Khalid Anwer Abdullah A. Aldossari Mohammed M. Alanazi Sary Alsanea Mohammed A. Assiri |
| author_sort | Naif O. Al-Harbi |
| collection | DOAJ |
| description | Lung injuries are attributed due to exposure to Drugs or chemicals. One of the important challenging situations for the clinicians is to manage treatments of different diseases with acute lung injury (ALI). The objective of this study was to investigate the possible protective mechanisms and action of a novel Phosphodiesterase-4 inhibitor “Apremilast” (AP) in lipopolysaccharide (LPS)-induced lung injury. Blood sample from each animals were collected in a vacuum blood collection tube. The rat lungs were isolated for oxidative stress assessment, western blot analysis and their mRNA expressions using RT-PCR. Exposure of LPS in rats causes significant increase in oxidative stress, activates the pro-inflammatory cytokines release like tissue necrotic factor-alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), modulated gene expression, protein expression and histopathological changes which were reversed by administration of AP. Finding of the research enlighten the protective role of AP against LPS-induced ALI. |
| first_indexed | 2024-04-11T17:35:15Z |
| format | Article |
| id | doaj.art-124206e3c366484d934533bdc36da47f |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-04-11T17:35:15Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-124206e3c366484d934533bdc36da47f2022-12-22T04:11:39ZengElsevierSaudi Journal of Biological Sciences1319-562X2022-05-0129534143424Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammationNaif O. Al-Harbi0Faisal Imam1Mohammad Matar Al-Harbi2Khaldoon Aljeryan3Othman A. Al-Shabanah4Khaled A. Alhosaini5Lamya Saif Alqahtani6Muhammad Afzal7M.D. Khalid Anwer8Abdullah A. Aldossari9Mohammed M. Alanazi10Sary Alsanea11Mohammed A. Assiri12Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Corresponding author.Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pathology, College of Medicine, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Cardiology, Prince Sultan Cardiac Center, Riyadh, Saudi Arabia, P.O. Box 99911, Riyadh 11625Department of Pharmacology, College of Pharmacy, Jouf University, Sakaka, Al Jouf 72341, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaLung injuries are attributed due to exposure to Drugs or chemicals. One of the important challenging situations for the clinicians is to manage treatments of different diseases with acute lung injury (ALI). The objective of this study was to investigate the possible protective mechanisms and action of a novel Phosphodiesterase-4 inhibitor “Apremilast” (AP) in lipopolysaccharide (LPS)-induced lung injury. Blood sample from each animals were collected in a vacuum blood collection tube. The rat lungs were isolated for oxidative stress assessment, western blot analysis and their mRNA expressions using RT-PCR. Exposure of LPS in rats causes significant increase in oxidative stress, activates the pro-inflammatory cytokines release like tissue necrotic factor-alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), modulated gene expression, protein expression and histopathological changes which were reversed by administration of AP. Finding of the research enlighten the protective role of AP against LPS-induced ALI.http://www.sciencedirect.com/science/article/pii/S1319562X22000997Acute lung injuryLipopolysaccharidesApremilastOxidative stressmRNA expressions |
| spellingShingle | Naif O. Al-Harbi Faisal Imam Mohammad Matar Al-Harbi Khaldoon Aljeryan Othman A. Al-Shabanah Khaled A. Alhosaini Lamya Saif Alqahtani Muhammad Afzal M.D. Khalid Anwer Abdullah A. Aldossari Mohammed M. Alanazi Sary Alsanea Mohammed A. Assiri Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation Saudi Journal of Biological Sciences Acute lung injury Lipopolysaccharides Apremilast Oxidative stress mRNA expressions |
| title | Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation |
| title_full | Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation |
| title_fullStr | Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation |
| title_full_unstemmed | Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation |
| title_short | Protective effect of Apremilast against LPS-induced acute lung injury via modulation of oxidative stress and inflammation |
| title_sort | protective effect of apremilast against lps induced acute lung injury via modulation of oxidative stress and inflammation |
| topic | Acute lung injury Lipopolysaccharides Apremilast Oxidative stress mRNA expressions |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X22000997 |
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