Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07)
Abstract Background While surgical resection remains the primary treatment approach for symptomatic or growing meningiomas, radiotherapy represents an auspicious alternative in patients with meningiomas not safely amenable to surgery. Biopsies are often omitted in light of potential postoperative ne...
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BMC
2024-04-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-024-12170-4 |
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| author | Maximilian Y. Deng Amanda Salviano da Silva Pauline Carlotta Göller Laila König Henning Schäfer Cecile Maire Adriane Lentz-Hommertgen Thomas Held Sebastian Regnery Tanja Eichkorn Florian Stritzke Lukas Bauer Daniel Schnell Klaus Herfarth Andreas von Deimling Sandro Krieg Antje Wick Wolfgang Wick Anca Grosu Jürgen Debus Felix Sahm Franz Ricklefs |
| author_facet | Maximilian Y. Deng Amanda Salviano da Silva Pauline Carlotta Göller Laila König Henning Schäfer Cecile Maire Adriane Lentz-Hommertgen Thomas Held Sebastian Regnery Tanja Eichkorn Florian Stritzke Lukas Bauer Daniel Schnell Klaus Herfarth Andreas von Deimling Sandro Krieg Antje Wick Wolfgang Wick Anca Grosu Jürgen Debus Felix Sahm Franz Ricklefs |
| author_sort | Maximilian Y. Deng |
| collection | DOAJ |
| description | Abstract Background While surgical resection remains the primary treatment approach for symptomatic or growing meningiomas, radiotherapy represents an auspicious alternative in patients with meningiomas not safely amenable to surgery. Biopsies are often omitted in light of potential postoperative neurological deficits, resulting in a lack of histological grading and (molecular) risk stratification. In this prospective explorative biomarker study, extracellular vesicles in the bloodstream will be investigated in patients with macroscopic meningiomas to identify a biomarker for molecular risk stratification and disease monitoring. Methods In total, 60 patients with meningiomas and an indication of radiotherapy (RT) and macroscopic tumor on the planning MRI will be enrolled. Blood samples will be obtained before the start, during, and after radiotherapy, as well as during clinical follow-up every 6 months. Extracellular vesicles will be isolated from the blood samples, quantified and correlated with the clinical treatment response or progression. Further, nanopore sequencing-based DNA methylation profiles of plasma EV-DNA will be generated for methylation-based meningioma classification. Discussion This study will explore the dynamic of plasma EVs in meningioma patients under/after radiotherapy, with the objective of identifying potential biomarkers of (early) tumor progression. DNA methylation profiling of plasma EVs in meningioma patients may enable molecular risk stratification, facilitating a molecularly-guided target volume delineation and adjusted dose prescription during RT treatment planning. |
| first_indexed | 2024-04-24T09:51:49Z |
| format | Article |
| id | doaj.art-124417bf66874d069f25f2e3934a9ba2 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-24T09:51:49Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-124417bf66874d069f25f2e3934a9ba22024-04-14T11:18:52ZengBMCBMC Cancer1471-24072024-04-012411610.1186/s12885-024-12170-4Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07)Maximilian Y. Deng0Amanda Salviano da Silva1Pauline Carlotta Göller2Laila König3Henning Schäfer4Cecile Maire5Adriane Lentz-Hommertgen6Thomas Held7Sebastian Regnery8Tanja Eichkorn9Florian Stritzke10Lukas Bauer11Daniel Schnell12Klaus Herfarth13Andreas von Deimling14Sandro Krieg15Antje Wick16Wolfgang Wick17Anca Grosu18Jürgen Debus19Felix Sahm20Franz Ricklefs21Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Neurosurgery, University Medical Center Hamburg-EppendorfDepartment of Neuropathology, CCU Neuropathology, Heidelberg University Hospital, Heidelberg University, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ)Department of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, University Medical Center Freiburg, University of FreiburgDepartment of Neurosurgery, University Medical Center Hamburg-EppendorfDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Radiation Oncology, University Medical Center Freiburg, University of FreiburgDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Neuropathology, CCU Neuropathology, Heidelberg University Hospital, Heidelberg University, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ)Department of Neurosurgery, University Hospital Heidelberg, Heidelberg UniversityNational Center for Tumor Diseases (NCT), NCT Heidelberg, a partnership between DKFZ and Heidelberg University HospitalNational Center for Tumor Diseases (NCT), NCT Heidelberg, a partnership between DKFZ and Heidelberg University HospitalDepartment of Radiation Oncology, University Medical Center Freiburg, University of FreiburgDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg UniversityDepartment of Neuropathology, CCU Neuropathology, Heidelberg University Hospital, Heidelberg University, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ)Department of Neurosurgery, University Medical Center Hamburg-EppendorfAbstract Background While surgical resection remains the primary treatment approach for symptomatic or growing meningiomas, radiotherapy represents an auspicious alternative in patients with meningiomas not safely amenable to surgery. Biopsies are often omitted in light of potential postoperative neurological deficits, resulting in a lack of histological grading and (molecular) risk stratification. In this prospective explorative biomarker study, extracellular vesicles in the bloodstream will be investigated in patients with macroscopic meningiomas to identify a biomarker for molecular risk stratification and disease monitoring. Methods In total, 60 patients with meningiomas and an indication of radiotherapy (RT) and macroscopic tumor on the planning MRI will be enrolled. Blood samples will be obtained before the start, during, and after radiotherapy, as well as during clinical follow-up every 6 months. Extracellular vesicles will be isolated from the blood samples, quantified and correlated with the clinical treatment response or progression. Further, nanopore sequencing-based DNA methylation profiles of plasma EV-DNA will be generated for methylation-based meningioma classification. Discussion This study will explore the dynamic of plasma EVs in meningioma patients under/after radiotherapy, with the objective of identifying potential biomarkers of (early) tumor progression. DNA methylation profiling of plasma EVs in meningioma patients may enable molecular risk stratification, facilitating a molecularly-guided target volume delineation and adjusted dose prescription during RT treatment planning.https://doi.org/10.1186/s12885-024-12170-4RadiotherapyLiquid biopsyExtracellular vesiclesMeningioma. |
| spellingShingle | Maximilian Y. Deng Amanda Salviano da Silva Pauline Carlotta Göller Laila König Henning Schäfer Cecile Maire Adriane Lentz-Hommertgen Thomas Held Sebastian Regnery Tanja Eichkorn Florian Stritzke Lukas Bauer Daniel Schnell Klaus Herfarth Andreas von Deimling Sandro Krieg Antje Wick Wolfgang Wick Anca Grosu Jürgen Debus Felix Sahm Franz Ricklefs Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) BMC Cancer Radiotherapy Liquid biopsy Extracellular vesicles Meningioma. |
| title | Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) |
| title_full | Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) |
| title_fullStr | Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) |
| title_full_unstemmed | Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) |
| title_short | Plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy- a prospective explorative biomarker study (ARO 2023-05/AG-NRO-07) |
| title_sort | plasma extracellular vesicles in meningioma patients following radiotherapy as liquid biopsy a prospective explorative biomarker study aro 2023 05 ag nro 07 |
| topic | Radiotherapy Liquid biopsy Extracellular vesicles Meningioma. |
| url | https://doi.org/10.1186/s12885-024-12170-4 |
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