Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line
<h2>Background: Luteinizing hormone (LH) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. The application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively.</h2><p><strong>M</st...
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2016-10-01
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| Series: | Novelty in Biomedicine |
| Subjects: | |
| Online Access: | http://journals.sbmu.ac.ir/nbm/article/view/11305 |
| _version_ | 1811288652086509568 |
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| author | Zeinab Soleimanifar Mojgan Bandehpour Bahram Kazemi |
| author_facet | Zeinab Soleimanifar Mojgan Bandehpour Bahram Kazemi |
| author_sort | Zeinab Soleimanifar |
| collection | DOAJ |
| description | <h2>Background: Luteinizing hormone (LH) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. The application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>In the present study the alpha and beta subunits of human LH gene were cloned into the pEGFP-N1 expression vector and produced the recombinant LH hormone in Chinese hamster ovary (CHO) eukaryotic system.</p><p><strong>Results</strong><strong>: </strong>Alpha and beta subunits of LH hormone were cloned between <em>Nhe</em>I and <em>BamH</em>I cut sites of pEGFP_N1 expression plasmid and confirmed by PCR. Hormone expression was evaluated in CHO cell line by Western blotting using the specific antibody.</p><p><strong>Conclusion: </strong>Alpha and beta subunits of LH hormone were expressed in CHO cell line perfectly.</p> |
| first_indexed | 2024-04-13T03:39:57Z |
| format | Article |
| id | doaj.art-125130ba488d47a3acabce890580cf96 |
| institution | Directory Open Access Journal |
| issn | 2345-3346 2345-3907 |
| language | English |
| last_indexed | 2024-04-13T03:39:57Z |
| publishDate | 2016-10-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Novelty in Biomedicine |
| spelling | doaj.art-125130ba488d47a3acabce890580cf962022-12-22T03:04:10ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072016-10-01441421467830Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell LineZeinab Soleimanifar0Mojgan Bandehpour1Bahram Kazemi2Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical SciencesDepartment of Biotechnology ,School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesDepartment of Biotechnology ,School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences<h2>Background: Luteinizing hormone (LH) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. The application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>In the present study the alpha and beta subunits of human LH gene were cloned into the pEGFP-N1 expression vector and produced the recombinant LH hormone in Chinese hamster ovary (CHO) eukaryotic system.</p><p><strong>Results</strong><strong>: </strong>Alpha and beta subunits of LH hormone were cloned between <em>Nhe</em>I and <em>BamH</em>I cut sites of pEGFP_N1 expression plasmid and confirmed by PCR. Hormone expression was evaluated in CHO cell line by Western blotting using the specific antibody.</p><p><strong>Conclusion: </strong>Alpha and beta subunits of LH hormone were expressed in CHO cell line perfectly.</p>http://journals.sbmu.ac.ir/nbm/article/view/11305LH, Amenorrhea, Sperm, cloning, Recombinant Hormone |
| spellingShingle | Zeinab Soleimanifar Mojgan Bandehpour Bahram Kazemi Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line Novelty in Biomedicine LH, Amenorrhea, Sperm, cloning, Recombinant Hormone |
| title | Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line |
| title_full | Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line |
| title_fullStr | Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line |
| title_full_unstemmed | Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line |
| title_short | Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line |
| title_sort | cloning and expression of luteinizing hormone subunits in chinese hamster ovary cell line |
| topic | LH, Amenorrhea, Sperm, cloning, Recombinant Hormone |
| url | http://journals.sbmu.ac.ir/nbm/article/view/11305 |
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