Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities
Probiotics have been applied to a wide range of bacteria, causing gastrointestinal and vaginal infections. However, probiotics generally possess limited antimicrobial spectra and are primarily utilized as dietary supplements. Recognizing the need for more versatile probiotics, this study focuses on...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024045122 |
| _version_ | 1827299243407179776 |
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| author | Jae Won Han Nari Lee Hea Joon Kim Sung Jin Moon Soo Chan Lee Hyo Jin Kim |
| author_facet | Jae Won Han Nari Lee Hea Joon Kim Sung Jin Moon Soo Chan Lee Hyo Jin Kim |
| author_sort | Jae Won Han |
| collection | DOAJ |
| description | Probiotics have been applied to a wide range of bacteria, causing gastrointestinal and vaginal infections. However, probiotics generally possess limited antimicrobial spectra and are primarily utilized as dietary supplements. Recognizing the need for more versatile probiotics, this study focuses on isolating and characterizing strains suitable for antibiotic replacement. Among these strains, Weissella sp. SNUL2, derived from a traditional fermented food in Korea (i.e., Sikhae), emerged as a promising candidate. The correlation between optical density at 600 nm and colony-forming units was verified and applied in subsequent experiments. To assess the therapeutic potential of probiotics, antibacterial tests were conducted using a microplate reader to evaluate the inhibition of 60 bacterial strains (including common foodborne pathogens) induced by Weissella sp. SNUL2 cell-free supernatant (CFS). The results confirmed its broad-spectrum antibacterial properties compared to previously known probiotics. Furthermore, enzymatic treatment with proteinases (trypsin and pepsin) and a time-kill assay were conducted to elucidate the nature of the antibacterial substance in Weissella sp. SNUL2 CFS. Through sequential chromatography involving gel filtration and ion-exchange chromatography, specific fractions with enhanced antibacterial properties were identified. LC-MS/MS analysis of the secretome fraction revealed the presence of various proteins from the C39 family, peptidoglycan endopeptidases, and N-acetylmuramoyl-l-alanine amidase domain-containing protein precursors. Hence, the combined action of these proteins may contribute to Weissella sp. SNUL2's broad antimicrobial activity. |
| first_indexed | 2024-04-24T15:30:33Z |
| format | Article |
| id | doaj.art-1256810af91944e588709cdb3d5312eb |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T15:30:33Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1256810af91944e588709cdb3d5312eb2024-04-02T04:15:18ZengElsevierHeliyon2405-84402024-04-01107e28481Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activitiesJae Won Han0Nari Lee1Hea Joon Kim2Sung Jin Moon3Soo Chan Lee4Hyo Jin Kim5Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of KoreaFood Safety and Distribution Research Group, Research Division of Strategic Food Technology, Korea Food Research Institute, Wanju-gun 55365, Republic of KoreaGraduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of KoreaDepartment of Internal Medicine, International St. Mary's Hospital, Catholic Kwandong University, Incheon 22711, Republic of KoreaSouth Texas Center of Emerging Infectious Diseases (STCEID), Department of Molecular Microbiology and Immunology, University of Texas at San Antonio, San Antonio, TX 78249, USAGraduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Corresponding author. Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.Probiotics have been applied to a wide range of bacteria, causing gastrointestinal and vaginal infections. However, probiotics generally possess limited antimicrobial spectra and are primarily utilized as dietary supplements. Recognizing the need for more versatile probiotics, this study focuses on isolating and characterizing strains suitable for antibiotic replacement. Among these strains, Weissella sp. SNUL2, derived from a traditional fermented food in Korea (i.e., Sikhae), emerged as a promising candidate. The correlation between optical density at 600 nm and colony-forming units was verified and applied in subsequent experiments. To assess the therapeutic potential of probiotics, antibacterial tests were conducted using a microplate reader to evaluate the inhibition of 60 bacterial strains (including common foodborne pathogens) induced by Weissella sp. SNUL2 cell-free supernatant (CFS). The results confirmed its broad-spectrum antibacterial properties compared to previously known probiotics. Furthermore, enzymatic treatment with proteinases (trypsin and pepsin) and a time-kill assay were conducted to elucidate the nature of the antibacterial substance in Weissella sp. SNUL2 CFS. Through sequential chromatography involving gel filtration and ion-exchange chromatography, specific fractions with enhanced antibacterial properties were identified. LC-MS/MS analysis of the secretome fraction revealed the presence of various proteins from the C39 family, peptidoglycan endopeptidases, and N-acetylmuramoyl-l-alanine amidase domain-containing protein precursors. Hence, the combined action of these proteins may contribute to Weissella sp. SNUL2's broad antimicrobial activity.http://www.sciencedirect.com/science/article/pii/S2405844024045122Weissella sp. SNUL2Antimicrobial peptideAntibiotic resistanceKorean traditional fermented foodProbioticsSecreted proteins |
| spellingShingle | Jae Won Han Nari Lee Hea Joon Kim Sung Jin Moon Soo Chan Lee Hyo Jin Kim Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities Heliyon Weissella sp. SNUL2 Antimicrobial peptide Antibiotic resistance Korean traditional fermented food Probiotics Secreted proteins |
| title | Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities |
| title_full | Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities |
| title_fullStr | Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities |
| title_full_unstemmed | Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities |
| title_short | Weissella sp. SNUL2 as potential probiotics with broad-spectrum antimicrobial activities |
| title_sort | weissella sp snul2 as potential probiotics with broad spectrum antimicrobial activities |
| topic | Weissella sp. SNUL2 Antimicrobial peptide Antibiotic resistance Korean traditional fermented food Probiotics Secreted proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024045122 |
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