Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment
<i>Cudrania tricuspidata</i> has diverse biological activities, such as antioxidant, anti-inflammatory, anticancer, and neuroprotective effects. This study investigated the protective effects of <i>C. tricuspidata</i> fruit extracts (CTFE) against scopolamine (SCO)-induced ne...
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9202 |
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| author | Seung-Cheol Jee Kwang Min Lee Min Kim Yoo-Jung Lee Soee Kim Joon-Oh Park Jung-Suk Sung |
| author_facet | Seung-Cheol Jee Kwang Min Lee Min Kim Yoo-Jung Lee Soee Kim Joon-Oh Park Jung-Suk Sung |
| author_sort | Seung-Cheol Jee |
| collection | DOAJ |
| description | <i>Cudrania tricuspidata</i> has diverse biological activities, such as antioxidant, anti-inflammatory, anticancer, and neuroprotective effects. This study investigated the protective effects of <i>C. tricuspidata</i> fruit extracts (CTFE) against scopolamine (SCO)-induced neuron impairment. The neuroprotective effects of CTFE on SCO-induced memory dysfunction were confirmed in mice using the Barnes maze test. The results showed that co-treatment of SCO and CTFE increased the stay time in the target zone compared with SCO treatment alone. Similarly, the results obtained by the fear conditioning test revealed that SCO-CTFE co-treatment induced the freezing action time under both the contextual fear condition and the cued fear condition compared with SCO treatment alone. Moreover, we showed that CTFE reduced the SCO-induced acetylcholinesterase (AChE) activity, thereby increasing the acetylcholine concentration in mice hippocampal tissues. Consistent with the improvement of memory and recognition function in vivo, our in vitro results showed that CTFE induced cAMP response element binding protein (CREB) and extracellular regulated kinase 1/2 (ERK1/2) activity in PC12 cells and reduced SCO-induced AChE activity. In addition, the microarray results of the hippocampal tissue support our data showing that CTFE affects gene expressions associated with neurogenesis and neuronal cell differentiation markers such as <i>spp1</i> and <i>klk6</i>. Overall, CTFE exerts a neuroprotective effect via regulation of the CREB and ERK1/2 signaling pathways and could be a therapeutic candidate for neurodegenerative diseases. |
| first_indexed | 2024-03-10T14:22:02Z |
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| language | English |
| last_indexed | 2024-03-10T14:22:02Z |
| publishDate | 2020-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-12572476609a467f9a505b9a3bcb16062023-11-20T23:16:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012123920210.3390/ijms21239202Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory ImpairmentSeung-Cheol Jee0Kwang Min Lee1Min Kim2Yoo-Jung Lee3Soee Kim4Joon-Oh Park5Jung-Suk Sung6Department of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, KoreaDepartment of Life Science, Biomedi Campus, Dongguk University-Seoul, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, Korea<i>Cudrania tricuspidata</i> has diverse biological activities, such as antioxidant, anti-inflammatory, anticancer, and neuroprotective effects. This study investigated the protective effects of <i>C. tricuspidata</i> fruit extracts (CTFE) against scopolamine (SCO)-induced neuron impairment. The neuroprotective effects of CTFE on SCO-induced memory dysfunction were confirmed in mice using the Barnes maze test. The results showed that co-treatment of SCO and CTFE increased the stay time in the target zone compared with SCO treatment alone. Similarly, the results obtained by the fear conditioning test revealed that SCO-CTFE co-treatment induced the freezing action time under both the contextual fear condition and the cued fear condition compared with SCO treatment alone. Moreover, we showed that CTFE reduced the SCO-induced acetylcholinesterase (AChE) activity, thereby increasing the acetylcholine concentration in mice hippocampal tissues. Consistent with the improvement of memory and recognition function in vivo, our in vitro results showed that CTFE induced cAMP response element binding protein (CREB) and extracellular regulated kinase 1/2 (ERK1/2) activity in PC12 cells and reduced SCO-induced AChE activity. In addition, the microarray results of the hippocampal tissue support our data showing that CTFE affects gene expressions associated with neurogenesis and neuronal cell differentiation markers such as <i>spp1</i> and <i>klk6</i>. Overall, CTFE exerts a neuroprotective effect via regulation of the CREB and ERK1/2 signaling pathways and could be a therapeutic candidate for neurodegenerative diseases.https://www.mdpi.com/1422-0067/21/23/9202<i>Cudrania tricuspidata</i>scopolamineCREBERK1/2learning and memory |
| spellingShingle | Seung-Cheol Jee Kwang Min Lee Min Kim Yoo-Jung Lee Soee Kim Joon-Oh Park Jung-Suk Sung Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment International Journal of Molecular Sciences <i>Cudrania tricuspidata</i> scopolamine CREB ERK1/2 learning and memory |
| title | Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment |
| title_full | Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment |
| title_fullStr | Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment |
| title_full_unstemmed | Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment |
| title_short | Neuroprotective Effect of <i>Cudrania tricuspidata</i> Fruit Extracts on Scopolamine-Induced Learning and Memory Impairment |
| title_sort | neuroprotective effect of i cudrania tricuspidata i fruit extracts on scopolamine induced learning and memory impairment |
| topic | <i>Cudrania tricuspidata</i> scopolamine CREB ERK1/2 learning and memory |
| url | https://www.mdpi.com/1422-0067/21/23/9202 |
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