Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation
The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous det...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal do Rio Grande do Sul
2021-06-01
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| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/dar/article/view/111454 |
| _version_ | 1797271645556047872 |
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| author | Morgana Souza Marques Fernando Dal Pont Morisso Fernanda Poletto Irene Clemes Külkamp Guerreiro |
| author_facet | Morgana Souza Marques Fernando Dal Pont Morisso Fernanda Poletto Irene Clemes Külkamp Guerreiro |
| author_sort | Morgana Souza Marques |
| collection | DOAJ |
| description |
The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocrossing point, the wavelength 247 nm and 365 nm were selected for PYZ and RIF quantification, respectively. No interference from cubosome excipients was detected in the proposed method. The results demonstrated linearity in a range of 4.0 – 12.0 µg/mL with an adequate correlation coefficient for both drugs. The intra and inter-day precision results (RSD < 5%) indicated the reproducibility of the method. The accuracy data showed satisfactory results (RSD < 5%) from recovery test. In addition, the robustness results showed that the PYZ and RIF content were unaffected by the solvent alteration of methanol to methanol:water (99:1, v/v). The derivative ultraviolet spectrophotometric method proved to be an excellent strategy for simultaneous determination of PYZ and RIF.
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| first_indexed | 2024-03-07T14:08:28Z |
| format | Article |
| id | doaj.art-1257f5733b244c9a9f9bd9ca031a2301 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-07T14:08:28Z |
| publishDate | 2021-06-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-1257f5733b244c9a9f9bd9ca031a23012024-03-06T18:15:03ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162021-06-0151Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulationMorgana Souza Marques0Fernando Dal Pont Morisso1Fernanda Poletto2Irene Clemes Külkamp Guerreiro3Universidade Federal do Rio Grande do SulFeevaleUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do Sul The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocrossing point, the wavelength 247 nm and 365 nm were selected for PYZ and RIF quantification, respectively. No interference from cubosome excipients was detected in the proposed method. The results demonstrated linearity in a range of 4.0 – 12.0 µg/mL with an adequate correlation coefficient for both drugs. The intra and inter-day precision results (RSD < 5%) indicated the reproducibility of the method. The accuracy data showed satisfactory results (RSD < 5%) from recovery test. In addition, the robustness results showed that the PYZ and RIF content were unaffected by the solvent alteration of methanol to methanol:water (99:1, v/v). The derivative ultraviolet spectrophotometric method proved to be an excellent strategy for simultaneous determination of PYZ and RIF. https://seer.ufrgs.br/index.php/dar/article/view/111454 |
| spellingShingle | Morgana Souza Marques Fernando Dal Pont Morisso Fernanda Poletto Irene Clemes Külkamp Guerreiro Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation Drug Analytical Research |
| title | Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| title_full | Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| title_fullStr | Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| title_full_unstemmed | Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| title_short | Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| title_sort | development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation |
| url | https://seer.ufrgs.br/index.php/dar/article/view/111454 |
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