Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer
Indoleamine 2,3-dioxygenase (IDO), a catabolizing enzyme of tryptophan, is supposed to play a role in tumor immune escape. Its expression in solid tumors has not yet been well elucidated: IDO can be expressed by the tumor cells themselves, or by ill-defined infiltrating cells, possibly depending on...
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| Format: | Article |
| Language: | English |
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Elsevier
2005-04-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558605800703 |
| _version_ | 1818938744888098816 |
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| author | Simonetta Astigiano Barbara Morandi Roberta Costa Luca Mastracci Antonella D'Agostino Giovanni Battista Ratto Giovanni Melioli Guido Frumento |
| author_facet | Simonetta Astigiano Barbara Morandi Roberta Costa Luca Mastracci Antonella D'Agostino Giovanni Battista Ratto Giovanni Melioli Guido Frumento |
| author_sort | Simonetta Astigiano |
| collection | DOAJ |
| description | Indoleamine 2,3-dioxygenase (IDO), a catabolizing enzyme of tryptophan, is supposed to play a role in tumor immune escape. Its expression in solid tumors has not yet been well elucidated: IDO can be expressed by the tumor cells themselves, or by ill-defined infiltrating cells, possibly depending on tumor type. We have investigated IDO expression in 25 cases of non small cell lung cancer (NSCLC). Using histochemistry and immunohistochemistry, we found that IDO was expressed not by tumor cells, but by normal cells infiltrating the peritumoral stroma. These cells were neither macrophages nor dendritic cells, and were identified as eosinophil granulocytes. The amount of IDO-positive eosinophils varied in different cases, ranging from a few cells to more than 50 per field at x200 magnification. IDO protein in NSCLC was enzymatically active. Therefore, at least in NSCLC cases displaying a large amount of these cells in the inflammatory infiltrate, IDO-positive eosinophils could exert an effective immunosuppressive action. On analyzing the 17 patients with adequate follow-up, a significant relationship was found between the amount of IDO-positive infiltrate and overall survival. This finding suggests that the degree of IDO-positive infiltrate could be a prognostic marker in NSCLC. |
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| format | Article |
| id | doaj.art-12599b9b5b1c44258e48d440e9a7f6dd |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-20T06:12:44Z |
| publishDate | 2005-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-12599b9b5b1c44258e48d440e9a7f6dd2022-12-21T19:50:37ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022005-04-017439039610.1593/neo.04658Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung CancerSimonetta Astigiano0Barbara Morandi1Roberta Costa2Luca Mastracci3Antonella D'Agostino4Giovanni Battista Ratto5Giovanni Melioli6Guido Frumento7Istituto Nazionale per la Ricerca sul Cancro, Genoa, ItalyIstituto Nazionale per la Ricerca sul Cancro, Genoa, ItalyChirurgia Toracica, Ospedale S. Antonio e Biagio e Cesare Arrigo, Alessandria, ItalyDICMI, Università di Genova, Genoa, ItalyLaboratori Centrali, Istituto G. Gaslini, Genoa, ItalyChirurgia Toracica, Ospedale S. Croce e Carle, Cuneo, ItalyLaboratori Centrali, Istituto G. Gaslini, Genoa, ItalyIstituto Nazionale per la Ricerca sul Cancro, Genoa, ItalyIndoleamine 2,3-dioxygenase (IDO), a catabolizing enzyme of tryptophan, is supposed to play a role in tumor immune escape. Its expression in solid tumors has not yet been well elucidated: IDO can be expressed by the tumor cells themselves, or by ill-defined infiltrating cells, possibly depending on tumor type. We have investigated IDO expression in 25 cases of non small cell lung cancer (NSCLC). Using histochemistry and immunohistochemistry, we found that IDO was expressed not by tumor cells, but by normal cells infiltrating the peritumoral stroma. These cells were neither macrophages nor dendritic cells, and were identified as eosinophil granulocytes. The amount of IDO-positive eosinophils varied in different cases, ranging from a few cells to more than 50 per field at x200 magnification. IDO protein in NSCLC was enzymatically active. Therefore, at least in NSCLC cases displaying a large amount of these cells in the inflammatory infiltrate, IDO-positive eosinophils could exert an effective immunosuppressive action. On analyzing the 17 patients with adequate follow-up, a significant relationship was found between the amount of IDO-positive infiltrate and overall survival. This finding suggests that the degree of IDO-positive infiltrate could be a prognostic marker in NSCLC.http://www.sciencedirect.com/science/article/pii/S1476558605800703Non small cell lung cancerindoleamine 2,3-dioxygenaseeosinophil granulocytesimmune escapeprognostic marker |
| spellingShingle | Simonetta Astigiano Barbara Morandi Roberta Costa Luca Mastracci Antonella D'Agostino Giovanni Battista Ratto Giovanni Melioli Guido Frumento Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer Neoplasia: An International Journal for Oncology Research Non small cell lung cancer indoleamine 2,3-dioxygenase eosinophil granulocytes immune escape prognostic marker |
| title | Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer |
| title_full | Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer |
| title_fullStr | Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer |
| title_full_unstemmed | Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer |
| title_short | Eosinophil Granulocytes Account for Indoleamine 2,3-Dioxygenase-Mediated Immune Escape in Human Non Small Cell Lung Cancer |
| title_sort | eosinophil granulocytes account for indoleamine 2 3 dioxygenase mediated immune escape in human non small cell lung cancer |
| topic | Non small cell lung cancer indoleamine 2,3-dioxygenase eosinophil granulocytes immune escape prognostic marker |
| url | http://www.sciencedirect.com/science/article/pii/S1476558605800703 |
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