Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples
Context: Giardiasis is one of the most common nonviral infections causing diarrheal illness worldwide. In this prospective cross-sectional study, we evaluated the RIDASCREEN ® Giardia kit for detection of Giardia intestinalis in stool samples and compared the results with direct microscopy. Material...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=1;spage=69;epage=71;aulast=Singhal |
| _version_ | 1819230866292867072 |
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| author | Shipra Singhal Vineeta Mittal Vineeta Khare Yengkokpam Ibotomba Singh |
| author_facet | Shipra Singhal Vineeta Mittal Vineeta Khare Yengkokpam Ibotomba Singh |
| author_sort | Shipra Singhal |
| collection | DOAJ |
| description | Context: Giardiasis is one of the most common nonviral infections causing diarrheal illness worldwide. In this prospective cross-sectional study, we evaluated the RIDASCREEN ® Giardia kit for detection of Giardia intestinalis in stool samples and compared the results with direct microscopy. Materials and methods: A total of 360 fecal samples were collected. They were then processed by wet film, iodine preparation and an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of Giardia trophozoites and cysts. Statistical analysis was performed by sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy. Results and Conclusion: Of the 360 cases, 17.2% samples were positive for Giardia by direct microscopy and 23.6% were found to be positive by ELISA (sensitivity ~97%), but specificity was ~92% only. Because of less specificity, we need to perform ELISA in congruence with direct microscopy, etc. Further studies need to be performed on a larger sample size using other molecular tests in order to get more accurate estimations. |
| first_indexed | 2024-12-23T11:35:52Z |
| format | Article |
| id | doaj.art-125a3ec040a240518fe6d47120a9d2cf |
| institution | Directory Open Access Journal |
| issn | 0377-4929 |
| language | English |
| last_indexed | 2024-12-23T11:35:52Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj.art-125a3ec040a240518fe6d47120a9d2cf2022-12-21T17:48:39ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292015-01-01581697110.4103/0377-4929.151192Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samplesShipra SinghalVineeta MittalVineeta KhareYengkokpam Ibotomba SinghContext: Giardiasis is one of the most common nonviral infections causing diarrheal illness worldwide. In this prospective cross-sectional study, we evaluated the RIDASCREEN ® Giardia kit for detection of Giardia intestinalis in stool samples and compared the results with direct microscopy. Materials and methods: A total of 360 fecal samples were collected. They were then processed by wet film, iodine preparation and an enzyme-linked immunosorbent assay (ELISA) kit to determine the presence of Giardia trophozoites and cysts. Statistical analysis was performed by sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy. Results and Conclusion: Of the 360 cases, 17.2% samples were positive for Giardia by direct microscopy and 23.6% were found to be positive by ELISA (sensitivity ~97%), but specificity was ~92% only. Because of less specificity, we need to perform ELISA in congruence with direct microscopy, etc. Further studies need to be performed on a larger sample size using other molecular tests in order to get more accurate estimations.http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=1;spage=69;epage=71;aulast=SinghalDirect microscopyenzyme-linked immunosorbent assayGiardia intestinalis |
| spellingShingle | Shipra Singhal Vineeta Mittal Vineeta Khare Yengkokpam Ibotomba Singh Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples Indian Journal of Pathology and Microbiology Direct microscopy enzyme-linked immunosorbent assay Giardia intestinalis |
| title | Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples |
| title_full | Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples |
| title_fullStr | Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples |
| title_full_unstemmed | Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples |
| title_short | Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples |
| title_sort | comparative analysis of enzyme linked immunosorbent assay and direct microscopy for the diagnosis of giardia intestinalis in fecal samples |
| topic | Direct microscopy enzyme-linked immunosorbent assay Giardia intestinalis |
| url | http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=1;spage=69;epage=71;aulast=Singhal |
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