Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
Summary: Ribosomes are composed of a mix of ribosomal RNAs and proteins; this composition varies depending on time, condition, and organism. Here, we present an optimized protocol for preparation of intact ribosomes from the skeletal muscle of the turquoise killifish. We also detail the steps for ri...
Main Authors: | Seongsin Lee, Yumi Kim |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007930 |
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