High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes
Alterations in the composition of the gut microbiota is thought to play a key role in causing type 2 diabetes, yet is not fully understood, especially at the strain level. Here, we used long-read DNA sequencing technology of 16S-ITS-23S rRNA genes for high-resolution characterization of gut microbio...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/758 |
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| author | Sarah A. Hendricks Chantal A. Vella Daniel D. New Afiya Aunjum Maximilian Antush Rayme Geidl Kimberly R. Andrews Onesmo B. Balemba |
| author_facet | Sarah A. Hendricks Chantal A. Vella Daniel D. New Afiya Aunjum Maximilian Antush Rayme Geidl Kimberly R. Andrews Onesmo B. Balemba |
| author_sort | Sarah A. Hendricks |
| collection | DOAJ |
| description | Alterations in the composition of the gut microbiota is thought to play a key role in causing type 2 diabetes, yet is not fully understood, especially at the strain level. Here, we used long-read DNA sequencing technology of 16S-ITS-23S rRNA genes for high-resolution characterization of gut microbiota in the development of type 2 diabetes. Gut microbiota composition was characterized from fecal DNA from 47 participants divided into 4 cohorts based on glycemic control: normal glycemic control (healthy; <i>n</i> = 21), reversed prediabetes (prediabetes/healthy; <i>n</i> = 8), prediabetes (<i>n</i> = 8), or type 2 diabetes (<i>n</i> = 10). A total of 46 taxa were found to be possibly related to progression from healthy state to type 2 diabetes. <i>Bacteroides coprophilus</i> DSM 18228, <i>Bifidobacterium pseudocatenulatum</i> DSM 20438, and <i>Bifidobacterium adolescentis</i> ATCC 15703 could confer resistance to glucose intolerance. On the other hand, <i>Odoribacter laneus</i> YIT 12061 may be pathogenic as it was found to be more abundant in type 2 diabetes participants than other cohorts. This research increases our understanding of the structural modulation of gut microbiota in the pathogenesis of type 2 diabetes and highlights gut microbiota strains, with the potential for targeted opportunistic pathogen control or consideration for probiotic prophylaxis and treatment. |
| first_indexed | 2024-03-11T06:08:58Z |
| format | Article |
| id | doaj.art-125deeba0c6e4db88c1501ed7cfe60a1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T06:08:58Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-125deeba0c6e4db88c1501ed7cfe60a12023-11-17T12:45:40ZengMDPI AGMicroorganisms2076-26072023-03-0111375810.3390/microorganisms11030758High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 DiabetesSarah A. Hendricks0Chantal A. Vella1Daniel D. New2Afiya Aunjum3Maximilian Antush4Rayme Geidl5Kimberly R. Andrews6Onesmo B. Balemba7Institute for Interdisciplinary Data Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Movement Sciences, University of Idaho, Moscow, ID 83843, USAInstitute for Interdisciplinary Data Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Movement Sciences, University of Idaho, Moscow, ID 83843, USADepartment of Movement Sciences, University of Idaho, Moscow, ID 83843, USAWWAMI Medical Education Program, University of Idaho, Moscow, ID 83843, USAInstitute for Interdisciplinary Data Sciences, University of Idaho, Moscow, ID 83843, USAWWAMI Medical Education Program, University of Idaho, Moscow, ID 83843, USAAlterations in the composition of the gut microbiota is thought to play a key role in causing type 2 diabetes, yet is not fully understood, especially at the strain level. Here, we used long-read DNA sequencing technology of 16S-ITS-23S rRNA genes for high-resolution characterization of gut microbiota in the development of type 2 diabetes. Gut microbiota composition was characterized from fecal DNA from 47 participants divided into 4 cohorts based on glycemic control: normal glycemic control (healthy; <i>n</i> = 21), reversed prediabetes (prediabetes/healthy; <i>n</i> = 8), prediabetes (<i>n</i> = 8), or type 2 diabetes (<i>n</i> = 10). A total of 46 taxa were found to be possibly related to progression from healthy state to type 2 diabetes. <i>Bacteroides coprophilus</i> DSM 18228, <i>Bifidobacterium pseudocatenulatum</i> DSM 20438, and <i>Bifidobacterium adolescentis</i> ATCC 15703 could confer resistance to glucose intolerance. On the other hand, <i>Odoribacter laneus</i> YIT 12061 may be pathogenic as it was found to be more abundant in type 2 diabetes participants than other cohorts. This research increases our understanding of the structural modulation of gut microbiota in the pathogenesis of type 2 diabetes and highlights gut microbiota strains, with the potential for targeted opportunistic pathogen control or consideration for probiotic prophylaxis and treatment.https://www.mdpi.com/2076-2607/11/3/758fecal microbiotagut microbiotahyperglycemiainsulin resistancelong-read sequencingprediabetes |
| spellingShingle | Sarah A. Hendricks Chantal A. Vella Daniel D. New Afiya Aunjum Maximilian Antush Rayme Geidl Kimberly R. Andrews Onesmo B. Balemba High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes Microorganisms fecal microbiota gut microbiota hyperglycemia insulin resistance long-read sequencing prediabetes |
| title | High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes |
| title_full | High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes |
| title_fullStr | High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes |
| title_full_unstemmed | High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes |
| title_short | High-Resolution Taxonomic Characterization Reveals Novel Human Microbial Strains with Potential as Risk Factors and Probiotics for Prediabetes and Type 2 Diabetes |
| title_sort | high resolution taxonomic characterization reveals novel human microbial strains with potential as risk factors and probiotics for prediabetes and type 2 diabetes |
| topic | fecal microbiota gut microbiota hyperglycemia insulin resistance long-read sequencing prediabetes |
| url | https://www.mdpi.com/2076-2607/11/3/758 |
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