LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia
Xiudong Chen, Qing Liu, Juan Chen, Yuhai Liu Department of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang City, Shandong Province, 277100, People’s Republic of ChinaCorrespondence: Yuhai LiuDepartment of Pediatric, Zaozhuang Maternal and Child Health Care Hospital,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-03-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/lncrna-rp11-248e95-and-rp11-456d71-are-valuable-for-the-diagnosis-of-c-peer-reviewed-article-IJGM |
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| author | Chen X Liu Q Chen J Liu Y |
| author_facet | Chen X Liu Q Chen J Liu Y |
| author_sort | Chen X |
| collection | DOAJ |
| description | Xiudong Chen, Qing Liu, Juan Chen, Yuhai Liu Department of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang City, Shandong Province, 277100, People’s Republic of ChinaCorrespondence: Yuhai LiuDepartment of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, No. 25, Wenhua East Road, Shizhong District, Zaozhuang City, Shandong Province, 277100, People’s Republic of ChinaTel +86-13969417606Email liuyuhai2001860@163.comBackground: Pneumonia is a common infection of the lung parenchyma in children, and early and accurate diagnosis of childhood pneumonia (CP) is important for implementing appropriate preventive and treatment strategies. This study aimed to evaluate the diagnostic value of the combination of long non-coding RNA (lncRNA) RP11-248E9.5, RP11-456D7.1, c-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in CP.Patients and Methods: A total of 50 healthy children (HC) and 100 CP patients were enrolled. The serum expression of RP11-248e9.5 and RP11-456d7.1 was detected by qRT-PCR. The white blood cell (WBC), hemoglobin (HB), platelet (PLT), neutrophil, and lymphocyte were analyzed by automated hematology analyzer. The serum levels of CRP and procalcitonin (PCT) were analyzed by automatic biochemical analyzer. The receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic value in CP.Results: The NLR and PLR, expression of RP11-248E9.5 and RP11-456D7.1, and serum levels of CRP and PCT were significantly higher in the CP group than those in the HC group. Both RP11-248E9.5 (AUC, 0.86; sensitivity, 84%; specificity, 78%) and RP11-456D7.1 (AUC, 0.89; sensitivity, 79%; specificity, 92%) exhibited certain diagnostic value in CP. The diagnostic values of PCT, CRP, NLR and PLR in CP were limited by low sensitivity (≤ 71%). The combination of multiple indicators improved the diagnostic value. The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR had the best diagnostic value in CP (AUC, 0.992; Sensitivity, 0.97; Specificity, 0.99).Conclusion: The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR was a potential diagnostic strategy for CP.Keywords: childhood pneumonia, long non-coding RNA, C-reactive protein, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio |
| first_indexed | 2024-12-22T13:08:11Z |
| format | Article |
| id | doaj.art-125e2f4193ee4336ac55101f2c010653 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-12-22T13:08:11Z |
| publishDate | 2021-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-125e2f4193ee4336ac55101f2c0106532022-12-21T18:24:48ZengDove Medical PressInternational Journal of General Medicine1178-70742021-03-01Volume 1489590263144LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood PneumoniaChen XLiu QChen JLiu YXiudong Chen, Qing Liu, Juan Chen, Yuhai Liu Department of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang City, Shandong Province, 277100, People’s Republic of ChinaCorrespondence: Yuhai LiuDepartment of Pediatric, Zaozhuang Maternal and Child Health Care Hospital, No. 25, Wenhua East Road, Shizhong District, Zaozhuang City, Shandong Province, 277100, People’s Republic of ChinaTel +86-13969417606Email liuyuhai2001860@163.comBackground: Pneumonia is a common infection of the lung parenchyma in children, and early and accurate diagnosis of childhood pneumonia (CP) is important for implementing appropriate preventive and treatment strategies. This study aimed to evaluate the diagnostic value of the combination of long non-coding RNA (lncRNA) RP11-248E9.5, RP11-456D7.1, c-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in CP.Patients and Methods: A total of 50 healthy children (HC) and 100 CP patients were enrolled. The serum expression of RP11-248e9.5 and RP11-456d7.1 was detected by qRT-PCR. The white blood cell (WBC), hemoglobin (HB), platelet (PLT), neutrophil, and lymphocyte were analyzed by automated hematology analyzer. The serum levels of CRP and procalcitonin (PCT) were analyzed by automatic biochemical analyzer. The receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic value in CP.Results: The NLR and PLR, expression of RP11-248E9.5 and RP11-456D7.1, and serum levels of CRP and PCT were significantly higher in the CP group than those in the HC group. Both RP11-248E9.5 (AUC, 0.86; sensitivity, 84%; specificity, 78%) and RP11-456D7.1 (AUC, 0.89; sensitivity, 79%; specificity, 92%) exhibited certain diagnostic value in CP. The diagnostic values of PCT, CRP, NLR and PLR in CP were limited by low sensitivity (≤ 71%). The combination of multiple indicators improved the diagnostic value. The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR had the best diagnostic value in CP (AUC, 0.992; Sensitivity, 0.97; Specificity, 0.99).Conclusion: The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR was a potential diagnostic strategy for CP.Keywords: childhood pneumonia, long non-coding RNA, C-reactive protein, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratiohttps://www.dovepress.com/lncrna-rp11-248e95-and-rp11-456d71-are-valuable-for-the-diagnosis-of-c-peer-reviewed-article-IJGMchildhood pneumonialong non-coding rnac-reactive proteinneutrophil-to-lymphocyte ratioplatelet-to-lymphocyte ratio |
| spellingShingle | Chen X Liu Q Chen J Liu Y LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia International Journal of General Medicine childhood pneumonia long non-coding rna c-reactive protein neutrophil-to-lymphocyte ratio platelet-to-lymphocyte ratio |
| title | LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia |
| title_full | LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia |
| title_fullStr | LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia |
| title_full_unstemmed | LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia |
| title_short | LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia |
| title_sort | lncrna rp11 248e9 5 and rp11 456d7 1 are valuable for the diagnosis of childhood pneumonia |
| topic | childhood pneumonia long non-coding rna c-reactive protein neutrophil-to-lymphocyte ratio platelet-to-lymphocyte ratio |
| url | https://www.dovepress.com/lncrna-rp11-248e95-and-rp11-456d71-are-valuable-for-the-diagnosis-of-c-peer-reviewed-article-IJGM |
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