Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria
Abstract A bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s respo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-06-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01048-w |
| _version_ | 1828769878367010816 |
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| author | Sinead T. Morrin Geoffrey McCarthy Deirdre Kennedy Mariarosaria Marotta Jane A. Irwin Rita M. Hickey |
| author_facet | Sinead T. Morrin Geoffrey McCarthy Deirdre Kennedy Mariarosaria Marotta Jane A. Irwin Rita M. Hickey |
| author_sort | Sinead T. Morrin |
| collection | DOAJ |
| description | Abstract A bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifidobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/mL IgG resulting in a 43-fold increase in the adhesion of Bifidobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in significant increases in adhesion of the Bifidobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut. |
| first_indexed | 2024-12-11T13:56:54Z |
| format | Article |
| id | doaj.art-125e64c343fc4bee9d91b0b876d0dc24 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-11T13:56:54Z |
| publishDate | 2020-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-125e64c343fc4bee9d91b0b876d0dc242022-12-22T01:04:01ZengSpringerOpenAMB Express2191-08552020-06-0110111010.1186/s13568-020-01048-wImmunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteriaSinead T. Morrin0Geoffrey McCarthy1Deirdre Kennedy2Mariarosaria Marotta3Jane A. Irwin4Rita M. Hickey5Teagasc Food Research CentreTeagasc Food Research CentreTeagasc Food Research CentreTeagasc Food Research CentreVeterinary Sciences Centre, School of Veterinary Medicine, University College DublinTeagasc Food Research CentreAbstract A bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifidobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/mL IgG resulting in a 43-fold increase in the adhesion of Bifidobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in significant increases in adhesion of the Bifidobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut.http://link.springer.com/article/10.1186/s13568-020-01048-wImmunoglobulin GMilkBifidobacteriumGlycosylationAdhesion |
| spellingShingle | Sinead T. Morrin Geoffrey McCarthy Deirdre Kennedy Mariarosaria Marotta Jane A. Irwin Rita M. Hickey Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria AMB Express Immunoglobulin G Milk Bifidobacterium Glycosylation Adhesion |
| title | Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| title_full | Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| title_fullStr | Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| title_full_unstemmed | Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| title_short | Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| title_sort | immunoglobulin g from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria |
| topic | Immunoglobulin G Milk Bifidobacterium Glycosylation Adhesion |
| url | http://link.springer.com/article/10.1186/s13568-020-01048-w |
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