Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens
Background: Neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. The aim of this study was to evaluate a PCR-based assay for specific and rapid detection of N. meninigitidis in CSF specimens. Methods: Since April 2...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2010-12-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/3080 |
| _version_ | 1818880500280852480 |
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| author | M Qurbanalizadegan R Ranjbar RA Ataee M Hajia Z Goodarzi S Farshad N Jonaidi Jafari Y Panahi H Kohanzad M Rahbar H Ghadimi M Izadi |
| author_facet | M Qurbanalizadegan R Ranjbar RA Ataee M Hajia Z Goodarzi S Farshad N Jonaidi Jafari Y Panahi H Kohanzad M Rahbar H Ghadimi M Izadi |
| author_sort | M Qurbanalizadegan |
| collection | DOAJ |
| description | Background: Neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. The aim of this study was to evaluate a PCR-based assay for specific and rapid detection of N. meninigitidis in CSF specimens.
Methods: Since April 2002 to July 2006, 130 CSF specimens were collected from patients suspected of having bacterial meningitis. Bacterial isolation and identification was carried out according to the standard bacteriological methods. The PCR was used to amplify a 101bp fragment of capsular transport gene A (ctrA) of N. meningitidis.
Results: PCR yielded an amplified product with the expected size of 101 base pair fragment. Sensitivity test proved 500 ng of N. meningitidis DNA as the final detection limit and specificity test revealed no cross-reaction for a wide range of respiratory pathogenic organisms.
Conclusion: The PCR assay was more sensitive than the bacterial culturing. It might be possible to apply this procedure for rapid diagnosis of meningococci in clinical samples. |
| first_indexed | 2024-12-19T14:46:57Z |
| format | Article |
| id | doaj.art-125f4bb24b5242e2b16566ea6891f33a |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T14:46:57Z |
| publishDate | 2010-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-125f4bb24b5242e2b16566ea6891f33a2022-12-21T20:16:56ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932010-12-01394Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid SpecimensM Qurbanalizadegan0R Ranjbar1RA Ataee2M Hajia 3Z Goodarzi4S Farshad5N Jonaidi Jafari6Y Panahi7H Kohanzad8M Rahbar9H Ghadimi10M Izadi11 Background: Neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. The aim of this study was to evaluate a PCR-based assay for specific and rapid detection of N. meninigitidis in CSF specimens. Methods: Since April 2002 to July 2006, 130 CSF specimens were collected from patients suspected of having bacterial meningitis. Bacterial isolation and identification was carried out according to the standard bacteriological methods. The PCR was used to amplify a 101bp fragment of capsular transport gene A (ctrA) of N. meningitidis. Results: PCR yielded an amplified product with the expected size of 101 base pair fragment. Sensitivity test proved 500 ng of N. meningitidis DNA as the final detection limit and specificity test revealed no cross-reaction for a wide range of respiratory pathogenic organisms. Conclusion: The PCR assay was more sensitive than the bacterial culturing. It might be possible to apply this procedure for rapid diagnosis of meningococci in clinical samples.https://ijph.tums.ac.ir/index.php/ijph/article/view/3080Meningococcal meningitisPCRNeisseria meningitidisRapid Detection |
| spellingShingle | M Qurbanalizadegan R Ranjbar RA Ataee M Hajia Z Goodarzi S Farshad N Jonaidi Jafari Y Panahi H Kohanzad M Rahbar H Ghadimi M Izadi Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens Iranian Journal of Public Health Meningococcal meningitis PCR Neisseria meningitidis Rapid Detection |
| title | Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens |
| title_full | Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens |
| title_fullStr | Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens |
| title_full_unstemmed | Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens |
| title_short | Specific PCR Assay for Rapid and Direct Detection of Neisseria meningitidis in Cerebrospinal Fluid Specimens |
| title_sort | specific pcr assay for rapid and direct detection of neisseria meningitidis in cerebrospinal fluid specimens |
| topic | Meningococcal meningitis PCR Neisseria meningitidis Rapid Detection |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/3080 |
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