Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i>
The genome of <i>Streptomyces</i> encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an u...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/734 |
| _version_ | 1797568586442604544 |
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| author | Yawei Zhao Guoquan Li Yunliang Chen Yinhua Lu |
| author_facet | Yawei Zhao Guoquan Li Yunliang Chen Yinhua Lu |
| author_sort | Yawei Zhao |
| collection | DOAJ |
| description | The genome of <i>Streptomyces</i> encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an unexplored rich reservoir of natural compounds, which can be used to discover novel chemical compounds. To activate silent BGCs for NP discovery, two main strategies, including the induction of BGCs expression in native hosts and heterologous expression of BGCs in surrogate <i>Streptomyces</i> hosts, have been adopted, which normally requires genetic manipulation. So far, various genome editing technologies have been developed, which has markedly facilitated the activation of BGCs and NP overproduction in their native hosts, as well as in heterologous <i>Streptomyces</i> hosts. In this review, we summarize the challenges and recent advances in genome editing tools for <i>Streptomyces</i> genetic manipulation with a focus on editing tools based on clustered regularly interspaced short palindrome repeat (CRISPR)/CRISPR-associated protein (Cas) systems. Additionally, we discuss the future research focus, especially the development of endogenous CRISPR/Cas-based genome editing technologies in <i>Streptomyces</i>. |
| first_indexed | 2024-03-10T19:58:06Z |
| format | Article |
| id | doaj.art-12602c2c648443e69dc1404e5b83246d |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:58:06Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-12602c2c648443e69dc1404e5b83246d2023-11-19T23:45:22ZengMDPI AGBiomolecules2218-273X2020-05-0110573410.3390/biom10050734Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i>Yawei Zhao0Guoquan Li1Yunliang Chen2Yinhua Lu3College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Agricultural Equipment Engineering, Zhenjiang, Jiangsu University Zhenjiang 212013, ChinaCollege of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaThe genome of <i>Streptomyces</i> encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an unexplored rich reservoir of natural compounds, which can be used to discover novel chemical compounds. To activate silent BGCs for NP discovery, two main strategies, including the induction of BGCs expression in native hosts and heterologous expression of BGCs in surrogate <i>Streptomyces</i> hosts, have been adopted, which normally requires genetic manipulation. So far, various genome editing technologies have been developed, which has markedly facilitated the activation of BGCs and NP overproduction in their native hosts, as well as in heterologous <i>Streptomyces</i> hosts. In this review, we summarize the challenges and recent advances in genome editing tools for <i>Streptomyces</i> genetic manipulation with a focus on editing tools based on clustered regularly interspaced short palindrome repeat (CRISPR)/CRISPR-associated protein (Cas) systems. Additionally, we discuss the future research focus, especially the development of endogenous CRISPR/Cas-based genome editing technologies in <i>Streptomyces</i>.https://www.mdpi.com/2218-273X/10/5/734genome editingCRISPR/Cas<i>Streptomyces</i>microbial natural products |
| spellingShingle | Yawei Zhao Guoquan Li Yunliang Chen Yinhua Lu Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> Biomolecules genome editing CRISPR/Cas <i>Streptomyces</i> microbial natural products |
| title | Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> |
| title_full | Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> |
| title_fullStr | Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> |
| title_full_unstemmed | Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> |
| title_short | Challenges and Advances in Genome Editing Technologies in <i>Streptomyces</i> |
| title_sort | challenges and advances in genome editing technologies in i streptomyces i |
| topic | genome editing CRISPR/Cas <i>Streptomyces</i> microbial natural products |
| url | https://www.mdpi.com/2218-273X/10/5/734 |
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