Piezo1 mutant zebrafish as a model of idiopathic scoliosis
Scoliosis is a condition where the spine curves sideways, unique to humans due to their upright posture. However, the cause of this disease is not well understood because it is challenging to find a model for experimentation. This study aimed to create a model for human idiopathic scoliosis by manip...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1321379/full |
| _version_ | 1827387634593300480 |
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| author | Ramli Toshihiro Aramaki Toshihiro Aramaki Masakatsu Watanabe Shigeru Kondo |
| author_facet | Ramli Toshihiro Aramaki Toshihiro Aramaki Masakatsu Watanabe Shigeru Kondo |
| author_sort | Ramli |
| collection | DOAJ |
| description | Scoliosis is a condition where the spine curves sideways, unique to humans due to their upright posture. However, the cause of this disease is not well understood because it is challenging to find a model for experimentation. This study aimed to create a model for human idiopathic scoliosis by manipulating the function of mechanosensitive channels called Piezo channels in zebrafish. Zebrafish were chosen because they experience similar biomechanical forces to humans, particularly in relation to the role of mechanical force in scoliosis progression. Here we describe piezo1 and piezo2a are involved in bone formation, with a double knockout resulting in congenital systemic malformations. However, an in-frame mutation of piezo1 led to fully penetrant juvenile-onset scoliosis, bone asymmetry, reduced tissue mineral density, and abnormal intervertebral discs—resembling non-congenital scoliosis symptoms in humans. These findings suggest that functional Piezo channels responding to mechanical forces are crucial for bone formation and maintaining spine integrity, providing insights into skeletal disorders. |
| first_indexed | 2024-03-08T16:05:08Z |
| format | Article |
| id | doaj.art-1263b5e24a2b4772a14cc50a3347635e |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-08T16:05:08Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-1263b5e24a2b4772a14cc50a3347635e2024-01-08T05:58:49ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-01-011410.3389/fgene.2023.13213791321379Piezo1 mutant zebrafish as a model of idiopathic scoliosis Ramli0Toshihiro Aramaki1Toshihiro Aramaki2Masakatsu Watanabe3Shigeru Kondo4Laboratory of Pattern Formation, Graduate School of Frontier Biosciences, Osaka University, Suita, JapanLaboratory of Pattern Formation, Graduate School of Frontier Biosciences, Osaka University, Suita, JapanJapan Science and Technology Agency, PRESTO, Tokyo, JapanLaboratory of Pattern Formation, Graduate School of Frontier Biosciences, Osaka University, Suita, JapanLaboratory of Pattern Formation, Graduate School of Frontier Biosciences, Osaka University, Suita, JapanScoliosis is a condition where the spine curves sideways, unique to humans due to their upright posture. However, the cause of this disease is not well understood because it is challenging to find a model for experimentation. This study aimed to create a model for human idiopathic scoliosis by manipulating the function of mechanosensitive channels called Piezo channels in zebrafish. Zebrafish were chosen because they experience similar biomechanical forces to humans, particularly in relation to the role of mechanical force in scoliosis progression. Here we describe piezo1 and piezo2a are involved in bone formation, with a double knockout resulting in congenital systemic malformations. However, an in-frame mutation of piezo1 led to fully penetrant juvenile-onset scoliosis, bone asymmetry, reduced tissue mineral density, and abnormal intervertebral discs—resembling non-congenital scoliosis symptoms in humans. These findings suggest that functional Piezo channels responding to mechanical forces are crucial for bone formation and maintaining spine integrity, providing insights into skeletal disorders.https://www.frontiersin.org/articles/10.3389/fgene.2023.1321379/fullidiopathic scoliosisPiezo channelTMDvertebral bonezebrafish |
| spellingShingle | Ramli Toshihiro Aramaki Toshihiro Aramaki Masakatsu Watanabe Shigeru Kondo Piezo1 mutant zebrafish as a model of idiopathic scoliosis Frontiers in Genetics idiopathic scoliosis Piezo channel TMD vertebral bone zebrafish |
| title | Piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| title_full | Piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| title_fullStr | Piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| title_full_unstemmed | Piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| title_short | Piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| title_sort | piezo1 mutant zebrafish as a model of idiopathic scoliosis |
| topic | idiopathic scoliosis Piezo channel TMD vertebral bone zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1321379/full |
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