Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective
The Arabidopsis AAA ATPase SKD1 is essential for ESCRT-dependent endosomal sorting by mediating the disassembly of the ESCRTIII complex in an ATP-dependent manner. In this study, we show that SKD1 localizes to messenger ribonucleoprotein complexes upon heat stress. Consistent with this, the interact...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.670499/full |
| _version_ | 1830476985882640384 |
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| author | Heike Wolff Marc Jakoby Lisa Stephan Eva Koebke Martin Hülskamp |
| author_facet | Heike Wolff Marc Jakoby Lisa Stephan Eva Koebke Martin Hülskamp |
| author_sort | Heike Wolff |
| collection | DOAJ |
| description | The Arabidopsis AAA ATPase SKD1 is essential for ESCRT-dependent endosomal sorting by mediating the disassembly of the ESCRTIII complex in an ATP-dependent manner. In this study, we show that SKD1 localizes to messenger ribonucleoprotein complexes upon heat stress. Consistent with this, the interactome of SKD1 revealed differential interactions under normal and stress conditions and included membrane transport proteins as well as proteins associated with RNA metabolism. Localization studies with selected interactome proteins revealed that not only RNA associated proteins but also several ESCRTIII and membrane trafficking proteins were recruited to messenger ribonucleoprotein granules after heat stress. |
| first_indexed | 2024-12-21T16:03:20Z |
| format | Article |
| id | doaj.art-1264963c6f3d42f6abdaa7e15717b6c2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T16:03:20Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1264963c6f3d42f6abdaa7e15717b6c22022-12-21T18:57:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.670499670499Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is SelectiveHeike Wolff0Marc Jakoby1Lisa Stephan2Eva Koebke3Martin Hülskamp4Cluster of Excellence on Plant Sciences (CEPLAS), Botanical Institute, Cologne University, Cologne, GermanyBotanical Institute, Biocenter, Cologne University, Cologne, GermanyBotanical Institute, Biocenter, Cologne University, Cologne, GermanyBotanical Institute, Biocenter, Cologne University, Cologne, GermanyBotanical Institute, Biocenter, Cologne University, Cologne, GermanyThe Arabidopsis AAA ATPase SKD1 is essential for ESCRT-dependent endosomal sorting by mediating the disassembly of the ESCRTIII complex in an ATP-dependent manner. In this study, we show that SKD1 localizes to messenger ribonucleoprotein complexes upon heat stress. Consistent with this, the interactome of SKD1 revealed differential interactions under normal and stress conditions and included membrane transport proteins as well as proteins associated with RNA metabolism. Localization studies with selected interactome proteins revealed that not only RNA associated proteins but also several ESCRTIII and membrane trafficking proteins were recruited to messenger ribonucleoprotein granules after heat stress.https://www.frontiersin.org/articles/10.3389/fpls.2021.670499/fullSKD1endosomal traffickingRNA metabolismmembrane traffickingESCRTheat stress |
| spellingShingle | Heike Wolff Marc Jakoby Lisa Stephan Eva Koebke Martin Hülskamp Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective Frontiers in Plant Science SKD1 endosomal trafficking RNA metabolism membrane trafficking ESCRT heat stress |
| title | Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective |
| title_full | Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective |
| title_fullStr | Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective |
| title_full_unstemmed | Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective |
| title_short | Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective |
| title_sort | heat stress dependent association of membrane trafficking proteins with mrnps is selective |
| topic | SKD1 endosomal trafficking RNA metabolism membrane trafficking ESCRT heat stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.670499/full |
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