| Summary: | In this research, tin ferrite (SnFe<sub>2</sub>O<sub>4</sub>) NPs were synthesized via hydrothermal route using ferric chloride and tin chloride as precursors and were then characterized in terms of morphology and structure using Fourier-transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV-Vis), X-ray power diffraction (XRD), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), and Brunauer–Emmett–Teller (BET) method. The obtained UV-Vis spectra was used to measure band gap energy of as-prepared SnFe<sub>2</sub>O<sub>4</sub> NPs. XRD confirmed the spinel structure of NPs, while SEM and TEM analyses disclosed the size of NPs in the range of 15–50 nm and revealed the spherical shape of NPs. Moreover, energy dispersive X-ray spectroscopy (EDS) and BET analysis was carried out to estimate elemental composition and specific surface area, respectively. In vitro cytotoxicity of the synthesized NPs were studied on normal (HUVEC, HEK293) and cancerous (A549) human cell lines. HUVEC cells were resistant to SnFe<sub>2</sub>O<sub>4</sub> NPs; while a significant decrease in the viability of HEK293 cells was observed when treated with higher concentrations of SnFe<sub>2</sub>O<sub>4</sub> NPs. Furthermore, SnFe<sub>2</sub>O<sub>4</sub> NPs induced dramatic cytotoxicity against A549 cells. For in vivo study, rats received SnFe<sub>2</sub>O<sub>4</sub> NPs at dosages of 0, 0.1, 1, and 10 mg/kg. The 10 mg/kg dose increased serum blood urea nitrogen and creatinine compared to the controls (P < 0.05). The pathology showed necrosis in the liver, heart, and lungs, and the greatest damages were related to the kidneys. Overall, the in vivo and in vitro experiments showed that SnFe<sub>2</sub>O<sub>4</sub> NPs at high doses had toxic effects on lung, liver and kidney cells without inducing toxicity to HUVECs. Further studies are warranted to fully elucidate the side effects of SnFe<sub>2</sub>O<sub>4</sub> NPs for their application in theranostics.
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