Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9
This dataset in brief is related to the research letter entitled “Nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9” [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize addition...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-10-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340919307504 |
| _version_ | 1818983837778051072 |
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| author | Julia Schachtsiek Felix Stehle |
| author_facet | Julia Schachtsiek Felix Stehle |
| author_sort | Julia Schachtsiek |
| collection | DOAJ |
| description | This dataset in brief is related to the research letter entitled “Nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9” [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize additional data and method descriptions of the generation process of a nicotine-free, nontransgenic tobacco plant. This included the cloning, transformation and regeneration of transgenic tobacco plants, followed by the analysis of the nicotine content and genomic modifications caused by CRISPR-Cas9 mediated gene editing. Subsequently, nicotine-free plants were screened for loss of T-DNA cassette, i.e. nontransgenity. Finally, a metabolic footprint was recorded by 1H NMR analysis. Keywords: CRISPR-Cas9, Nicotine-free, Gene editing, Nontransgenic, Nicotiana tabacum, Non-addictive |
| first_indexed | 2024-12-20T18:09:28Z |
| format | Article |
| id | doaj.art-126b8e75e6f141fca57d5fdef8666ce7 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-20T18:09:28Z |
| publishDate | 2019-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-126b8e75e6f141fca57d5fdef8666ce72022-12-21T19:30:30ZengElsevierData in Brief2352-34092019-10-0126Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9Julia Schachtsiek0Felix Stehle1Laboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, GermanyCorresponding author.; Laboratory of Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, GermanyThis dataset in brief is related to the research letter entitled “Nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9” [1]. Cured tobacco products with a significantly reduced nicotine content helps people to overcome their nicotine addiction. Here we summarize additional data and method descriptions of the generation process of a nicotine-free, nontransgenic tobacco plant. This included the cloning, transformation and regeneration of transgenic tobacco plants, followed by the analysis of the nicotine content and genomic modifications caused by CRISPR-Cas9 mediated gene editing. Subsequently, nicotine-free plants were screened for loss of T-DNA cassette, i.e. nontransgenity. Finally, a metabolic footprint was recorded by 1H NMR analysis. Keywords: CRISPR-Cas9, Nicotine-free, Gene editing, Nontransgenic, Nicotiana tabacum, Non-addictivehttp://www.sciencedirect.com/science/article/pii/S2352340919307504 |
| spellingShingle | Julia Schachtsiek Felix Stehle Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 Data in Brief |
| title | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
| title_full | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
| title_fullStr | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
| title_full_unstemmed | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
| title_short | Dataset on nicotine-free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR-Cas9 |
| title_sort | dataset on nicotine free nontransgenic tobacco nicotiana tabacum l edited by crispr cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S2352340919307504 |
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