Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different re...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-14894-w |
| _version_ | 1818751803905277952 |
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| author | Guillaume Tetreau Anne-Sophie Banneville Elena A. Andreeva Aaron S. Brewster Mark S. Hunter Raymond G. Sierra Jean-Marie Teulon Iris D. Young Niamh Burke Tilman A. Grünewald Joël Beaudouin Irina Snigireva Maria Teresa Fernandez-Luna Alister Burt Hyun-Woo Park Luca Signor Jayesh A. Bafna Rabia Sadir Daphna Fenel Elisabetta Boeri-Erba Maria Bacia Ninon Zala Frédéric Laporte Laurence Després Martin Weik Sébastien Boutet Martin Rosenthal Nicolas Coquelle Manfred Burghammer Duilio Cascio Michael R. Sawaya Mathias Winterhalter Enrico Gratton Irina Gutsche Brian Federici Jean-Luc Pellequer Nicholas K. Sauter Jacques-Philippe Colletier |
| author_facet | Guillaume Tetreau Anne-Sophie Banneville Elena A. Andreeva Aaron S. Brewster Mark S. Hunter Raymond G. Sierra Jean-Marie Teulon Iris D. Young Niamh Burke Tilman A. Grünewald Joël Beaudouin Irina Snigireva Maria Teresa Fernandez-Luna Alister Burt Hyun-Woo Park Luca Signor Jayesh A. Bafna Rabia Sadir Daphna Fenel Elisabetta Boeri-Erba Maria Bacia Ninon Zala Frédéric Laporte Laurence Després Martin Weik Sébastien Boutet Martin Rosenthal Nicolas Coquelle Manfred Burghammer Duilio Cascio Michael R. Sawaya Mathias Winterhalter Enrico Gratton Irina Gutsche Brian Federici Jean-Luc Pellequer Nicholas K. Sauter Jacques-Philippe Colletier |
| author_sort | Guillaume Tetreau |
| collection | DOAJ |
| description | Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade. |
| first_indexed | 2024-12-18T04:41:23Z |
| format | Article |
| id | doaj.art-126ca4694d8a47efbec919f942778d20 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T04:41:23Z |
| publishDate | 2020-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-126ca4694d8a47efbec919f942778d202022-12-21T21:20:43ZengNature PortfolioNature Communications2041-17232020-03-0111111610.1038/s41467-020-14894-wSerial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascadeGuillaume Tetreau0Anne-Sophie Banneville1Elena A. Andreeva2Aaron S. Brewster3Mark S. Hunter4Raymond G. Sierra5Jean-Marie Teulon6Iris D. Young7Niamh Burke8Tilman A. Grünewald9Joël Beaudouin10Irina Snigireva11Maria Teresa Fernandez-Luna12Alister Burt13Hyun-Woo Park14Luca Signor15Jayesh A. Bafna16Rabia Sadir17Daphna Fenel18Elisabetta Boeri-Erba19Maria Bacia20Ninon Zala21Frédéric Laporte22Laurence Després23Martin Weik24Sébastien Boutet25Martin Rosenthal26Nicolas Coquelle27Manfred Burghammer28Duilio Cascio29Michael R. Sawaya30Mathias Winterhalter31Enrico Gratton32Irina Gutsche33Brian Federici34Jean-Luc Pellequer35Nicholas K. Sauter36Jacques-Philippe Colletier37Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National LaboratoryLinac Coherent Light Source, SLAC National Accelerator LaboratoryLinac Coherent Light Source, SLAC National Accelerator LaboratoryUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National LaboratoryUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleEuropean Synchrotron Radiation Facility (ESRF), BP 220Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleEuropean Synchrotron Radiation Facility (ESRF), BP 220Department of Entomology and Institute for Integrative Genome Biology, University of CaliforniaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleDepartment of Entomology and Institute for Integrative Genome Biology, University of CaliforniaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleDepartment of Life Sciences & Chemistry, Jacobs UniversityUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleUniv. Grenoble Alpes, CNRS, LECAUniv. Grenoble Alpes, CNRS, LECAUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleLinac Coherent Light Source, SLAC National Accelerator LaboratoryEuropean Synchrotron Radiation Facility (ESRF), BP 220Large-Scale Structures Group, Institut Laue-LangevinEuropean Synchrotron Radiation Facility (ESRF), BP 220UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of CaliforniaUCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of CaliforniaUniv. Grenoble Alpes, CNRS, LECAThe Henry Samueli School of Engineering, University of CaliforniaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleDepartment of Entomology and Institute for Integrative Genome Biology, University of CaliforniaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National LaboratoryUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie StructuraleBacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade.https://doi.org/10.1038/s41467-020-14894-w |
| spellingShingle | Guillaume Tetreau Anne-Sophie Banneville Elena A. Andreeva Aaron S. Brewster Mark S. Hunter Raymond G. Sierra Jean-Marie Teulon Iris D. Young Niamh Burke Tilman A. Grünewald Joël Beaudouin Irina Snigireva Maria Teresa Fernandez-Luna Alister Burt Hyun-Woo Park Luca Signor Jayesh A. Bafna Rabia Sadir Daphna Fenel Elisabetta Boeri-Erba Maria Bacia Ninon Zala Frédéric Laporte Laurence Després Martin Weik Sébastien Boutet Martin Rosenthal Nicolas Coquelle Manfred Burghammer Duilio Cascio Michael R. Sawaya Mathias Winterhalter Enrico Gratton Irina Gutsche Brian Federici Jean-Luc Pellequer Nicholas K. Sauter Jacques-Philippe Colletier Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade Nature Communications |
| title | Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade |
| title_full | Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade |
| title_fullStr | Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade |
| title_full_unstemmed | Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade |
| title_short | Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade |
| title_sort | serial femtosecond crystallography on in vivo grown crystals drives elucidation of mosquitocidal cyt1aa bioactivation cascade |
| url | https://doi.org/10.1038/s41467-020-14894-w |
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