First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea
The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stem...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2022-09-01
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| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://online-rpd.org/upload/pdf/RPD-2022-28-3-172.pdf |
| _version_ | 1798020781594116096 |
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| author | Kyoung-Taek Park Soo-Min Hong Chang-Gi Back San Yeong Kim Seung-Yeol Lee In-Kyu Kang Leonid N. Ten Hee-Young Jung |
| author_facet | Kyoung-Taek Park Soo-Min Hong Chang-Gi Back San Yeong Kim Seung-Yeol Lee In-Kyu Kang Leonid N. Ten Hee-Young Jung |
| author_sort | Kyoung-Taek Park |
| collection | DOAJ |
| description | The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on t he results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea. |
| first_indexed | 2024-04-11T17:03:18Z |
| format | Article |
| id | doaj.art-126e49ec4e6648df88d5170cc64e03f9 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-04-11T17:03:18Z |
| publishDate | 2022-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-126e49ec4e6648df88d5170cc64e03f92022-12-22T04:13:07ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912022-09-0128317217810.5423/RPD.2022.28.3.1721757First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in KoreaKyoung-Taek Park0Soo-Min Hong1Chang-Gi Back2San Yeong Kim3Seung-Yeol Lee4In-Kyu Kang5Leonid N. Ten6Hee-Young Jung7 School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Gumi Floriculture Research Institute, Gyeongsangbuk-do Agricultural Research and Extension Services, Gumi 39102, Korea Gumi Floriculture Research Institute, Gyeongsangbuk-do Agricultural Research and Extension Services, Gumi 39102, Korea Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaThe graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on t he results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.http://online-rpd.org/upload/pdf/RPD-2022-28-3-172.pdfgraft cactusgymnocalycium mihanovichiipathogenicitypectobacterium brasiliensesoft rot |
| spellingShingle | Kyoung-Taek Park Soo-Min Hong Chang-Gi Back San Yeong Kim Seung-Yeol Lee In-Kyu Kang Leonid N. Ten Hee-Young Jung First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea Research in Plant Disease graft cactus gymnocalycium mihanovichii pathogenicity pectobacterium brasiliense soft rot |
| title | First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea |
| title_full | First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea |
| title_fullStr | First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea |
| title_full_unstemmed | First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea |
| title_short | First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea |
| title_sort | first report of pectobacterium brasiliense causing soft rot on graft cactus in korea |
| topic | graft cactus gymnocalycium mihanovichii pathogenicity pectobacterium brasiliense soft rot |
| url | http://online-rpd.org/upload/pdf/RPD-2022-28-3-172.pdf |
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