A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid
Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed...
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2024-03-01
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| author | Mihaela Răcuciu Simona Oancea Lucian Barbu-Tudoran Olga Drăghici Anda Agavriloaei Dorina Creangă |
| author_facet | Mihaela Răcuciu Simona Oancea Lucian Barbu-Tudoran Olga Drăghici Anda Agavriloaei Dorina Creangă |
| author_sort | Mihaela Răcuciu |
| collection | DOAJ |
| description | Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by the electrostatic adsorption of HA onto the nanoparticles’ surfaces. A theoretical study carried out with the PM3 method evidenced a dipole moment of 3.34 D and negatively charged atom groups able to participate in interactions with nanoparticle surface cations and surrounding water molecules. The ATR-FTIR spectrum evidenced the hyaluronic acid binding to the surface of the ferrophase, ensuring colloidal stability in the water dispersion. To verify the success of the synthesis and stabilization, HA-MNPs were also characterized using other investigation techniques: TEM, EDS, XRD, DSC, TG, NTA, and VSM. The results showed that the HA-MNPs had a mean physical size of 9.05 nm (TEM investigation), a crystallite dimension of about 8.35 nm (XRD investigation), and a magnetic core diameter of about 8.31 nm (VSM investigation). The HA-MNPs exhibited superparamagnetic behavior, with the magnetization curve showing saturation at a high magnetic field and a very small coercive field, corresponding to the net dominance of single-domain magnetic nanoparticles that were not aggregated with reversible magnetizability. These features satisfy the requirement for magnetic nanoparticles with a small size and good dispersibility for long-term stability. We performed some preliminary tests regarding the nanotoxicity in the environment, and some chromosomal aberrations were found to be induced in corn root meristems, especially in the anaphase and metaphase of mitotic cells. Due to their properties, HA-MNPs also seem to be suitable for use in the biomedical field. |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-24T18:03:37Z |
| publishDate | 2024-03-01 |
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| series | Materials |
| spelling | doaj.art-127023add6524eb88c7841de48cf4b862024-03-27T13:52:16ZengMDPI AGMaterials1996-19442024-03-01176122910.3390/ma17061229A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic AcidMihaela Răcuciu0Simona Oancea1Lucian Barbu-Tudoran2Olga Drăghici3Anda Agavriloaei4Dorina Creangă5Environmental Sciences and Physics Department, Faculty of Sciences, Lucian Blaga University of Sibiu, Dr. I. Ratiu Str., no. 5–7, 550012 Sibiu, RomaniaAgricultural Sciences and Food Engineering Department, Lucian Blaga University of Sibiu, Dr. I. Ratiu Str., no. 7–9, 550012 Sibiu, RomaniaElectron Microscopy Integrated Laboratory, National Institute for R&D of Isotopic and Molecular Technologies, Donat Str., no. 67-103, 400293 Cluj-Napoca, RomaniaAgricultural Sciences and Food Engineering Department, Lucian Blaga University of Sibiu, Dr. I. Ratiu Str., no. 7–9, 550012 Sibiu, RomaniaBiophysics and Medical Physics Laboratory, Faculty of Physics, “Alexandru Ioan Cuza” University, 11, Carol I Blvd., 700506 Iasi, RomaniaBiophysics and Medical Physics Laboratory, Faculty of Physics, “Alexandru Ioan Cuza” University, 11, Carol I Blvd., 700506 Iasi, RomaniaHyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by the electrostatic adsorption of HA onto the nanoparticles’ surfaces. A theoretical study carried out with the PM3 method evidenced a dipole moment of 3.34 D and negatively charged atom groups able to participate in interactions with nanoparticle surface cations and surrounding water molecules. The ATR-FTIR spectrum evidenced the hyaluronic acid binding to the surface of the ferrophase, ensuring colloidal stability in the water dispersion. To verify the success of the synthesis and stabilization, HA-MNPs were also characterized using other investigation techniques: TEM, EDS, XRD, DSC, TG, NTA, and VSM. The results showed that the HA-MNPs had a mean physical size of 9.05 nm (TEM investigation), a crystallite dimension of about 8.35 nm (XRD investigation), and a magnetic core diameter of about 8.31 nm (VSM investigation). The HA-MNPs exhibited superparamagnetic behavior, with the magnetization curve showing saturation at a high magnetic field and a very small coercive field, corresponding to the net dominance of single-domain magnetic nanoparticles that were not aggregated with reversible magnetizability. These features satisfy the requirement for magnetic nanoparticles with a small size and good dispersibility for long-term stability. We performed some preliminary tests regarding the nanotoxicity in the environment, and some chromosomal aberrations were found to be induced in corn root meristems, especially in the anaphase and metaphase of mitotic cells. Due to their properties, HA-MNPs also seem to be suitable for use in the biomedical field.https://www.mdpi.com/1996-1944/17/6/1229magnetic nanoparticleshyaluronic acidiron oxidenanoparticles’ sizebiomedical uses |
| spellingShingle | Mihaela Răcuciu Simona Oancea Lucian Barbu-Tudoran Olga Drăghici Anda Agavriloaei Dorina Creangă A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid Materials magnetic nanoparticles hyaluronic acid iron oxide nanoparticles’ size biomedical uses |
| title | A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid |
| title_full | A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid |
| title_fullStr | A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid |
| title_full_unstemmed | A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid |
| title_short | A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid |
| title_sort | study of hyaluronic acid s theoretical reactivity and of magnetic nanoparticles capped with hyaluronic acid |
| topic | magnetic nanoparticles hyaluronic acid iron oxide nanoparticles’ size biomedical uses |
| url | https://www.mdpi.com/1996-1944/17/6/1229 |
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