RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development
The development of floral organs is coordinated by an elaborate network of homeotic genes, and gibberellin (GA) signaling is involved in floral organ development; however, the underlying molecular mechanisms remain elusive. In the present study, we found that MOS4-ASSOCIATED COMPLEX 5A (MAC5A), whic...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/4/2009 |
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| author | Hua Liu Hongna Shang Huan Yang Wenjie Liu Daisuke Tsugama Ken-Ichi Nonomura Aimin Zhou Wenwu Wu Tetsuo Takano Shenkui Liu |
| author_facet | Hua Liu Hongna Shang Huan Yang Wenjie Liu Daisuke Tsugama Ken-Ichi Nonomura Aimin Zhou Wenwu Wu Tetsuo Takano Shenkui Liu |
| author_sort | Hua Liu |
| collection | DOAJ |
| description | The development of floral organs is coordinated by an elaborate network of homeotic genes, and gibberellin (GA) signaling is involved in floral organ development; however, the underlying molecular mechanisms remain elusive. In the present study, we found that MOS4-ASSOCIATED COMPLEX 5A (MAC5A), which is a protein containing an RNA-binding motif, was involved in the development of sepals, petals, and stamens; either the loss or gain of <i>MAC5A</i> function resulted in stamen malformation and a reduced seed set. The exogenous application of GA considerably exacerbated the defects in <i>mac5a</i> null mutants, including fewer stamens and male sterility. <i>MAC5A</i> was predominantly expressed in pollen grains and stamens, and overexpression of <i>MAC5A</i> affected the expression of homeotic genes such as <i>APETALA1</i> (<i>AP1</i>), <i>AP2</i>, and <i>AGAMOUS</i> (<i>AG</i>). MAC5A may interact with RABBIT EARS (RBE), a repressor of <i>AG</i> expression in <i>Arabidopsis</i> flowers. The petal defect in <i>rbe</i> null mutants was at least partly rescued in <i>mac5a rbe</i> double mutants. These findings suggest that <i>MAC5A</i> is a novel factor that is required for the normal development of stamens and depends on the GA signaling pathway. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:44:43Z |
| publishDate | 2022-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-12703a8bcab94213a4231a360f023cc22023-11-23T20:18:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234200910.3390/ijms23042009RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen DevelopmentHua Liu0Hongna Shang1Huan Yang2Wenjie Liu3Daisuke Tsugama4Ken-Ichi Nonomura5Aimin Zhou6Wenwu Wu7Tetsuo Takano8Shenkui Liu9State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaAsian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, JapanPlant Cytogenetics Laboratory, National Institute of Genetics (NIG), Shizuoka 411-0801, JapanCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaAsian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, JapanState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaThe development of floral organs is coordinated by an elaborate network of homeotic genes, and gibberellin (GA) signaling is involved in floral organ development; however, the underlying molecular mechanisms remain elusive. In the present study, we found that MOS4-ASSOCIATED COMPLEX 5A (MAC5A), which is a protein containing an RNA-binding motif, was involved in the development of sepals, petals, and stamens; either the loss or gain of <i>MAC5A</i> function resulted in stamen malformation and a reduced seed set. The exogenous application of GA considerably exacerbated the defects in <i>mac5a</i> null mutants, including fewer stamens and male sterility. <i>MAC5A</i> was predominantly expressed in pollen grains and stamens, and overexpression of <i>MAC5A</i> affected the expression of homeotic genes such as <i>APETALA1</i> (<i>AP1</i>), <i>AP2</i>, and <i>AGAMOUS</i> (<i>AG</i>). MAC5A may interact with RABBIT EARS (RBE), a repressor of <i>AG</i> expression in <i>Arabidopsis</i> flowers. The petal defect in <i>rbe</i> null mutants was at least partly rescued in <i>mac5a rbe</i> double mutants. These findings suggest that <i>MAC5A</i> is a novel factor that is required for the normal development of stamens and depends on the GA signaling pathway.https://www.mdpi.com/1422-0067/23/4/2009<i>Arabidopsis thaliana</i>floral organ developmentgibberellinstamenmale sterilityMAC5A |
| spellingShingle | Hua Liu Hongna Shang Huan Yang Wenjie Liu Daisuke Tsugama Ken-Ichi Nonomura Aimin Zhou Wenwu Wu Tetsuo Takano Shenkui Liu RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> floral organ development gibberellin stamen male sterility MAC5A |
| title | RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development |
| title_full | RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development |
| title_fullStr | RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development |
| title_full_unstemmed | RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development |
| title_short | RNA-Binding Protein MAC5A Is Required for Gibberellin-Regulated Stamen Development |
| title_sort | rna binding protein mac5a is required for gibberellin regulated stamen development |
| topic | <i>Arabidopsis thaliana</i> floral organ development gibberellin stamen male sterility MAC5A |
| url | https://www.mdpi.com/1422-0067/23/4/2009 |
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