A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB
Abstract Mycovirus‐mediated hypovirulence has the potential to control fungal diseases. However, the availability of hypovirulence‐conferring mycoviruses for plant fungal disease control is limited as most fungal viruses are asymptomatic. In this study, the virus‐induced gene silencing (VIGS) vector...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-10-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202302606 |
| _version_ | 1797657778013077504 |
|---|---|
| author | Lihang Zhang Shuangchao Wang Shaojian Ruan Clement Nzabanita Yanfei Wang Lihua Guo |
| author_facet | Lihang Zhang Shuangchao Wang Shaojian Ruan Clement Nzabanita Yanfei Wang Lihua Guo |
| author_sort | Lihang Zhang |
| collection | DOAJ |
| description | Abstract Mycovirus‐mediated hypovirulence has the potential to control fungal diseases. However, the availability of hypovirulence‐conferring mycoviruses for plant fungal disease control is limited as most fungal viruses are asymptomatic. In this study, the virus‐induced gene silencing (VIGS) vector p26‐D4 of Fusarium graminearum gemytripvirus 1 (FgGMTV1), a tripartite circular single‐stranded DNA mycovirus, is successfully constructed to convert the causal fungus of cereal Fusarium head blight (FHB) into a hypovirulent strain. p26‐D4, with an insert of a 75–150 bp fragment of the target reporter transgene transcript in both sense and antisense orientations, efficiently triggered gene silencing in Fusarium graminearum. Notably, the two hypovirulent strains, p26‐D4‐Tri101, and p26‐D4‐FgPP1, obtained by silencing the virulence‐related genes Tri101 and FgPP1 with p26‐D4, can be used as biocontrol agents to protect wheat from a fungal disease FHB and mycotoxin contamination at the field level. This study not only describes the first mycovirus‐derived VIGS system but also proves that the VIGS vector can be used to establish multiple hypovirulent strains to control pathogenic fungi. |
| first_indexed | 2024-03-11T17:50:39Z |
| format | Article |
| id | doaj.art-1275aa5d76f6423c9036fe16868b424a |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-11T17:50:39Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-1275aa5d76f6423c9036fe16868b424a2023-10-18T06:07:54ZengWileyAdvanced Science2198-38442023-10-011029n/an/a10.1002/advs.202302606A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHBLihang Zhang0Shuangchao Wang1Shaojian Ruan2Clement Nzabanita3Yanfei Wang4Lihua Guo5State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 ChinaAbstract Mycovirus‐mediated hypovirulence has the potential to control fungal diseases. However, the availability of hypovirulence‐conferring mycoviruses for plant fungal disease control is limited as most fungal viruses are asymptomatic. In this study, the virus‐induced gene silencing (VIGS) vector p26‐D4 of Fusarium graminearum gemytripvirus 1 (FgGMTV1), a tripartite circular single‐stranded DNA mycovirus, is successfully constructed to convert the causal fungus of cereal Fusarium head blight (FHB) into a hypovirulent strain. p26‐D4, with an insert of a 75–150 bp fragment of the target reporter transgene transcript in both sense and antisense orientations, efficiently triggered gene silencing in Fusarium graminearum. Notably, the two hypovirulent strains, p26‐D4‐Tri101, and p26‐D4‐FgPP1, obtained by silencing the virulence‐related genes Tri101 and FgPP1 with p26‐D4, can be used as biocontrol agents to protect wheat from a fungal disease FHB and mycotoxin contamination at the field level. This study not only describes the first mycovirus‐derived VIGS system but also proves that the VIGS vector can be used to establish multiple hypovirulent strains to control pathogenic fungi.https://doi.org/10.1002/advs.202302606FgGMTV1hypovirulencemycovirusVIGSwheat FHB |
| spellingShingle | Lihang Zhang Shuangchao Wang Shaojian Ruan Clement Nzabanita Yanfei Wang Lihua Guo A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB Advanced Science FgGMTV1 hypovirulence mycovirus VIGS wheat FHB |
| title | A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB |
| title_full | A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB |
| title_fullStr | A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB |
| title_full_unstemmed | A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB |
| title_short | A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB |
| title_sort | mycovirus vigs vector confers hypovirulence to a plant pathogenic fungus to control wheat fhb |
| topic | FgGMTV1 hypovirulence mycovirus VIGS wheat FHB |
| url | https://doi.org/10.1002/advs.202302606 |
| work_keys_str_mv | AT lihangzhang amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT shuangchaowang amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT shaojianruan amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT clementnzabanita amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT yanfeiwang amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT lihuaguo amycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT lihangzhang mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT shuangchaowang mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT shaojianruan mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT clementnzabanita mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT yanfeiwang mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb AT lihuaguo mycovirusvigsvectorconfershypovirulencetoaplantpathogenicfungustocontrolwheatfhb |