Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I
This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coup...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/5/448 |
| _version_ | 1797536828359704576 |
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| author | Vien Thi Tran Heongkyu Ju |
| author_facet | Vien Thi Tran Heongkyu Ju |
| author_sort | Vien Thi Tran |
| collection | DOAJ |
| description | This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL<sup>−1</sup> (atto-gram mL<sup>−1</sup>) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity. |
| first_indexed | 2024-03-10T12:07:19Z |
| format | Article |
| id | doaj.art-12761345eb5642d18ea8a58fe5648660 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T12:07:19Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-12761345eb5642d18ea8a58fe56486602023-11-21T16:29:43ZengMDPI AGBiomedicines2227-90592021-04-019544810.3390/biomedicines9050448Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin IVien Thi Tran0Heongkyu Ju1Department of Physics, Gachon University, Seongnam-si, Gyeonggi-do 13120, KoreaDepartment of Physics, Gachon University, Seongnam-si, Gyeonggi-do 13120, KoreaThis work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL<sup>−1</sup> (atto-gram mL<sup>−1</sup>) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.https://www.mdpi.com/2227-9059/9/5/448fluorescencecardiac biomarkerTroponin Isurface plasmon coupled emissionlab-on-chip optical biosensor |
| spellingShingle | Vien Thi Tran Heongkyu Ju Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I Biomedicines fluorescence cardiac biomarker Troponin I surface plasmon coupled emission lab-on-chip optical biosensor |
| title | Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I |
| title_full | Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I |
| title_fullStr | Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I |
| title_full_unstemmed | Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I |
| title_short | Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I |
| title_sort | fluorescence based on surface plasmon coupled emission for ultrahigh sensitivity immunoassay of cardiac troponin i |
| topic | fluorescence cardiac biomarker Troponin I surface plasmon coupled emission lab-on-chip optical biosensor |
| url | https://www.mdpi.com/2227-9059/9/5/448 |
| work_keys_str_mv | AT vienthitran fluorescencebasedonsurfaceplasmoncoupledemissionforultrahighsensitivityimmunoassayofcardiactroponini AT heongkyuju fluorescencebasedonsurfaceplasmoncoupledemissionforultrahighsensitivityimmunoassayofcardiactroponini |