Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice
Background: Korean Red Ginseng (KRG), the steamed root of Panax ginseng, has pharmacological activities for immunological and neurodegenerative disorders. But, the role of KRGE in multiple sclerosis (MS) remains unclear. Purpose: To determine whether KRG extract (KRGE) could inhibit demyelination in...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Ginseng Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845323000544 |
| _version_ | 1797733044359004160 |
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| author | Min Jung Lee Jong Hee Choi Tae Woo Kwon Hyo-Sung Jo Yujeong Ha Seung-Yeol Nah Ik-Hyun Cho |
| author_facet | Min Jung Lee Jong Hee Choi Tae Woo Kwon Hyo-Sung Jo Yujeong Ha Seung-Yeol Nah Ik-Hyun Cho |
| author_sort | Min Jung Lee |
| collection | DOAJ |
| description | Background: Korean Red Ginseng (KRG), the steamed root of Panax ginseng, has pharmacological activities for immunological and neurodegenerative disorders. But, the role of KRGE in multiple sclerosis (MS) remains unclear. Purpose: To determine whether KRG extract (KRGE) could inhibit demyelination in corpus callosum (CC) of cuprizone (CPZ)-induced murine model of MS Methods: Male adult mice were fed with a standard chow diet or a chow diet supplemented with 0.2% (w/w) CPZ ad libitum for six weeks to induce demyelination while were simultaneously administered with distilled water (DW) alone or KRGE-DW (0.004%, 0.02 and 0.1% of KRGE) by drinking. Results: Administration with KRGE-DW alleviated demyelination and oligodendrocyte degeneration associated with inhibition of infiltration and activation of resident microglia and monocyte-derived macrophages as well as downregulation of proinflammatory mediators in the CC of CPZ-fed mice. KRGE-DW also attenuated the level of infiltration of Th1 and Th17) cells, in line with inhibited mRNA expression of IFN-γ and IL-17, respectively, in the CC. These positive effects of KRGE-DW mitigated behavioral dysfunction based on elevated plus maze and the rotarod tests. Conclusion: The results strongly suggest that KRGE-DW may inhibit CPZ-induced demyelination due to its oligodendroglial protective and anti-inflammatory activities by inhibiting infiltration/activation of immune cells. Thus, KRGE might have potential in therapeutic intervention for MS. |
| first_indexed | 2024-03-12T12:23:21Z |
| format | Article |
| id | doaj.art-127c7bb5cd9941b590883878d6123839 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-03-12T12:23:21Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-127c7bb5cd9941b590883878d61238392023-08-30T05:50:09ZengElsevierJournal of Ginseng Research1226-84532023-09-01475672680Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated miceMin Jung Lee0Jong Hee Choi1Tae Woo Kwon2Hyo-Sung Jo3Yujeong Ha4Seung-Yeol Nah5Ik-Hyun Cho6Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea; Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, USADepartment of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of KoreaDepartment of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of KoreaGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, Republic of KoreaDepartment of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea; Corresponding author.Background: Korean Red Ginseng (KRG), the steamed root of Panax ginseng, has pharmacological activities for immunological and neurodegenerative disorders. But, the role of KRGE in multiple sclerosis (MS) remains unclear. Purpose: To determine whether KRG extract (KRGE) could inhibit demyelination in corpus callosum (CC) of cuprizone (CPZ)-induced murine model of MS Methods: Male adult mice were fed with a standard chow diet or a chow diet supplemented with 0.2% (w/w) CPZ ad libitum for six weeks to induce demyelination while were simultaneously administered with distilled water (DW) alone or KRGE-DW (0.004%, 0.02 and 0.1% of KRGE) by drinking. Results: Administration with KRGE-DW alleviated demyelination and oligodendrocyte degeneration associated with inhibition of infiltration and activation of resident microglia and monocyte-derived macrophages as well as downregulation of proinflammatory mediators in the CC of CPZ-fed mice. KRGE-DW also attenuated the level of infiltration of Th1 and Th17) cells, in line with inhibited mRNA expression of IFN-γ and IL-17, respectively, in the CC. These positive effects of KRGE-DW mitigated behavioral dysfunction based on elevated plus maze and the rotarod tests. Conclusion: The results strongly suggest that KRGE-DW may inhibit CPZ-induced demyelination due to its oligodendroglial protective and anti-inflammatory activities by inhibiting infiltration/activation of immune cells. Thus, KRGE might have potential in therapeutic intervention for MS.http://www.sciencedirect.com/science/article/pii/S1226845323000544Korean Red GinsengCuprizone-induced demyelinationOliogodendrocyte-protectionAnti-inflammation |
| spellingShingle | Min Jung Lee Jong Hee Choi Tae Woo Kwon Hyo-Sung Jo Yujeong Ha Seung-Yeol Nah Ik-Hyun Cho Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice Journal of Ginseng Research Korean Red Ginseng Cuprizone-induced demyelination Oliogodendrocyte-protection Anti-inflammation |
| title | Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice |
| title_full | Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice |
| title_fullStr | Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice |
| title_full_unstemmed | Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice |
| title_short | Korean Red Ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone-administrated mice |
| title_sort | korean red ginseng extract ameliorates demyelination by inhibiting infiltration and activation of immune cells in cuprizone administrated mice |
| topic | Korean Red Ginseng Cuprizone-induced demyelination Oliogodendrocyte-protection Anti-inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1226845323000544 |
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