<sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence
This study investigated metabolite changes in three pomelo cultivars during postharvest senescence using <sup>1</sup>H NMR-based metabolic profiling. Three pomelo cultivars, ‘Hongroumiyou’, ‘Bairoumiyou’ and ‘Huangroumiyou’, abbreviated as “R”, “W” and “Y” according to the color of their...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/10/2001 |
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| author | Juan Liu Xinqiao Zhou Dagang Chen Jie Guo Ke Chen Chanjuan Ye Chuanguang Liu |
| author_facet | Juan Liu Xinqiao Zhou Dagang Chen Jie Guo Ke Chen Chanjuan Ye Chuanguang Liu |
| author_sort | Juan Liu |
| collection | DOAJ |
| description | This study investigated metabolite changes in three pomelo cultivars during postharvest senescence using <sup>1</sup>H NMR-based metabolic profiling. Three pomelo cultivars, ‘Hongroumiyou’, ‘Bairoumiyou’ and ‘Huangroumiyou’, abbreviated as “R”, “W” and “Y” according to the color of their juice sacs, were stored at 25 °C for 90 days, and NMR was applied to determine the metabolite changes in juice sacs during storage. Fifteen metabolites were identified, including organic acids, sugars, amino acids, fatty acids, phenols and naringin. Partial least squares discriminant analysis (PLS-DA) was used to screen the significant metabolites according to the variable importance for the projection (VIP) scores in three pomelo cultivars during 90 days of storage. Additionally, eight metabolites, naringin, alanine, asparagine, choline, citric acid, malic acid, phosphocholine and β-D-glucose, were screened to be the crucial biomarkers with VIP > 1. The undesirable flavor of “bitter and sour” during the 60 days of storage was mainly attributed to the naringin, citric acid and sugars. According to the correlation analysis, the citric acid content determined by NMR showed a significantly positive relationship with that analyzed by HPLC. These findings suggested that NMR technology was accurate and efficient for metabolomic analysis of pomelo fruit, and the <sup>1</sup>H NMR-based metabolic profiling can be efficient during quality evaluation and useful for improving the fruit flavor quality during postharvest storage. |
| first_indexed | 2024-03-11T03:44:20Z |
| format | Article |
| id | doaj.art-127ce2de49ec4bd2a89c164fb2d538bd |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T03:44:20Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-127ce2de49ec4bd2a89c164fb2d538bd2023-11-18T01:21:31ZengMDPI AGFoods2304-81582023-05-011210200110.3390/foods12102001<sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest SenescenceJuan Liu0Xinqiao Zhou1Dagang Chen2Jie Guo3Ke Chen4Chanjuan Ye5Chuanguang Liu6Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Rice Engineering Laboratory, Guangdong Key Laboratory of New Technology in Rice Breeding, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Rice Engineering Laboratory, Guangzhou 510640, ChinaThis study investigated metabolite changes in three pomelo cultivars during postharvest senescence using <sup>1</sup>H NMR-based metabolic profiling. Three pomelo cultivars, ‘Hongroumiyou’, ‘Bairoumiyou’ and ‘Huangroumiyou’, abbreviated as “R”, “W” and “Y” according to the color of their juice sacs, were stored at 25 °C for 90 days, and NMR was applied to determine the metabolite changes in juice sacs during storage. Fifteen metabolites were identified, including organic acids, sugars, amino acids, fatty acids, phenols and naringin. Partial least squares discriminant analysis (PLS-DA) was used to screen the significant metabolites according to the variable importance for the projection (VIP) scores in three pomelo cultivars during 90 days of storage. Additionally, eight metabolites, naringin, alanine, asparagine, choline, citric acid, malic acid, phosphocholine and β-D-glucose, were screened to be the crucial biomarkers with VIP > 1. The undesirable flavor of “bitter and sour” during the 60 days of storage was mainly attributed to the naringin, citric acid and sugars. According to the correlation analysis, the citric acid content determined by NMR showed a significantly positive relationship with that analyzed by HPLC. These findings suggested that NMR technology was accurate and efficient for metabolomic analysis of pomelo fruit, and the <sup>1</sup>H NMR-based metabolic profiling can be efficient during quality evaluation and useful for improving the fruit flavor quality during postharvest storage.https://www.mdpi.com/2304-8158/12/10/2001pomelometabolic profilingNMRnaringincitric acidflavor |
| spellingShingle | Juan Liu Xinqiao Zhou Dagang Chen Jie Guo Ke Chen Chanjuan Ye Chuanguang Liu <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence Foods pomelo metabolic profiling NMR naringin citric acid flavor |
| title | <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence |
| title_full | <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence |
| title_fullStr | <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence |
| title_full_unstemmed | <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence |
| title_short | <sup>1</sup>H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence |
| title_sort | sup 1 sup h nmr based metabolic profiling to follow changes in pomelo cultivars during postharvest senescence |
| topic | pomelo metabolic profiling NMR naringin citric acid flavor |
| url | https://www.mdpi.com/2304-8158/12/10/2001 |
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