Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine
The recent development of bone-on-chips (BOCs) holds the main advantage of requiring a low quantity of cells and material, compared to traditional In Vitro models. By incorporating hydrogels within BOCs, the culture system moved to a three dimensional culture environment for cells which is more repr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/10/4495 |
| _version_ | 1797534078945198080 |
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| author | Gabriele Nasello Mar Cóndor Ted Vaughan Jessica Schiavi |
| author_facet | Gabriele Nasello Mar Cóndor Ted Vaughan Jessica Schiavi |
| author_sort | Gabriele Nasello |
| collection | DOAJ |
| description | The recent development of bone-on-chips (BOCs) holds the main advantage of requiring a low quantity of cells and material, compared to traditional In Vitro models. By incorporating hydrogels within BOCs, the culture system moved to a three dimensional culture environment for cells which is more representative of bone tissue matrix and function. The fundamental components of hydrogel-based BOCs, namely the cellular sources, the hydrogel and the culture chamber, have been tuned to mimic the hematopoietic niche in the bone aspirate marrow, cancer bone metastasis and osteo/chondrogenic differentiation. In this review, we examine the entire process of developing hydrogel-based BOCs to model In Vitro a patient specific situation. First, we provide bone biological understanding for BOCs design and then how hydrogel structural and mechanical properties can be tuned to meet those requirements. This is followed by a review on hydrogel-based BOCs, developed in the last 10 years, in terms of culture chamber design, hydrogel and cell source used. Finally, we provide guidelines for the definition of personalized pathological and physiological bone microenvironments. This review covers the information on bone, hydrogel and BOC that are required to develop personalized therapies for bone disease, by recreating clinically relevant scenarii in miniaturized devices. |
| first_indexed | 2024-03-10T11:24:32Z |
| format | Article |
| id | doaj.art-127deea804604a79ab5eac9231c87820 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T11:24:32Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-127deea804604a79ab5eac9231c878202023-11-21T19:45:40ZengMDPI AGApplied Sciences2076-34172021-05-011110449510.3390/app11104495Designing Hydrogel-Based Bone-On-Chips for Personalized MedicineGabriele Nasello0Mar Cóndor1Ted Vaughan2Jessica Schiavi3Multiscale in Mechanical and Biological Engineering (M2BE), University of Zaragoza, 50009 Zaragoza, SpainBiomechanics Section, Department of Mechanical Engineering, KU Leuven, 3000 Leuven, BelgiumDepartment of Biomedical Engineering, College of Engineering and Sciences, National University of Ireland, H91 HX31 Galway, IrelandDepartment of Biomedical Engineering, College of Engineering and Sciences, National University of Ireland, H91 HX31 Galway, IrelandThe recent development of bone-on-chips (BOCs) holds the main advantage of requiring a low quantity of cells and material, compared to traditional In Vitro models. By incorporating hydrogels within BOCs, the culture system moved to a three dimensional culture environment for cells which is more representative of bone tissue matrix and function. The fundamental components of hydrogel-based BOCs, namely the cellular sources, the hydrogel and the culture chamber, have been tuned to mimic the hematopoietic niche in the bone aspirate marrow, cancer bone metastasis and osteo/chondrogenic differentiation. In this review, we examine the entire process of developing hydrogel-based BOCs to model In Vitro a patient specific situation. First, we provide bone biological understanding for BOCs design and then how hydrogel structural and mechanical properties can be tuned to meet those requirements. This is followed by a review on hydrogel-based BOCs, developed in the last 10 years, in terms of culture chamber design, hydrogel and cell source used. Finally, we provide guidelines for the definition of personalized pathological and physiological bone microenvironments. This review covers the information on bone, hydrogel and BOC that are required to develop personalized therapies for bone disease, by recreating clinically relevant scenarii in miniaturized devices.https://www.mdpi.com/2076-3417/11/10/4495bone-on-chipbone microenvironmentbone hydrogelstunable hydrogelsmicrofluidic bone treatmentsbone models |
| spellingShingle | Gabriele Nasello Mar Cóndor Ted Vaughan Jessica Schiavi Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine Applied Sciences bone-on-chip bone microenvironment bone hydrogels tunable hydrogels microfluidic bone treatments bone models |
| title | Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine |
| title_full | Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine |
| title_fullStr | Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine |
| title_full_unstemmed | Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine |
| title_short | Designing Hydrogel-Based Bone-On-Chips for Personalized Medicine |
| title_sort | designing hydrogel based bone on chips for personalized medicine |
| topic | bone-on-chip bone microenvironment bone hydrogels tunable hydrogels microfluidic bone treatments bone models |
| url | https://www.mdpi.com/2076-3417/11/10/4495 |
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