Simultaneous Metabarcoding and Quantification of <i>Neocallimastigomycetes</i> from Environmental Samples: Insights into Community Composition and Novel Lineages

Simultaneous Metabarcoding and Quantification of <i>Neocallimastigomycetes</i> from Environmental Samples: Insights into Community Composition and Novel Lineages

Anaerobic fungi from the herbivore digestive tract (<i>Neocallimastigomycetes</i>) are primary lignocellulose modifiers and hold promise for biotechnological applications. Their molecular detection is currently difficult due to the non-specificity of published primer pairs, which impairs...

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Bibliographic Details
Main Authors: Diana Young, Akshay Joshi, Liren Huang, Bernhard Munk, Christian Wurzbacher, Noha H. Youssef, Mostafa S. Elshahed, Christina D. Moon, Katrin Ochsenreither, Gareth W. Griffith, Tony M. Callaghan, Alexander Sczyrba, Michael Lebuhn, Veronika Flad
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Microorganisms
Subjects:
anaerobic gut fungi
<i>Neocallimastigomycetes</i>
environmental screening
Quantitative Real-Time PCR
large ribosomal subunit
barcoding
Online Access:https://www.mdpi.com/2076-2607/10/9/1749

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