<i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus
The <i>Bacillus cereus</i> group of bacteria includes, inter alia, the species known to be associated with human diseases and food poisoning. Here, we describe the <i>Bacillus</i> phage vB_BtS_B83 (abbreviated as B83) infecting the species of this group. Transmission electron...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/7/624 |
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| author | Emma G. Piligrimova Olesya A. Kazantseva Nikita A. Nikulin Andrey M. Shadrin |
| author_facet | Emma G. Piligrimova Olesya A. Kazantseva Nikita A. Nikulin Andrey M. Shadrin |
| author_sort | Emma G. Piligrimova |
| collection | DOAJ |
| description | The <i>Bacillus cereus</i> group of bacteria includes, inter alia, the species known to be associated with human diseases and food poisoning. Here, we describe the <i>Bacillus</i> phage vB_BtS_B83 (abbreviated as B83) infecting the species of this group. Transmission electron microscopy (TEM) micrographs indicate that B83 belongs to the <i>Siphoviridae</i> family. B83 is a temperate phage using an arbitrium system for the regulation of the lysis−lysogeny switch, and is probably capable of forming a circular plasmid prophage. Comparative analysis shows that it has been previously sequenced, but was mistaken for a plasmid. B83 shares common genome organization and >46% of proteins with other the <i>Bacillus</i> phage, BMBtp14. Phylograms constructed using large terminase subunits and a pan-genome presence−absence matrix show that these phages form a clade distinct from the closest viruses. Based on the above, we propose the creation of a new genus named <i>Bembunaquatrovirus</i> that includes B83 and BMBtp14. |
| first_indexed | 2024-12-21T22:36:30Z |
| format | Article |
| id | doaj.art-1286b0948a864d5bbfb00ec9b47c7641 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T22:36:30Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1286b0948a864d5bbfb00ec9b47c76412022-12-21T18:47:57ZengMDPI AGViruses1999-49152019-07-0111762410.3390/v11070624v11070624<i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> GenusEmma G. Piligrimova0Olesya A. Kazantseva1Nikita A. Nikulin2Andrey M. Shadrin3Laboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, RussiaLaboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, RussiaLaboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, RussiaLaboratory of Bacteriophage Biology, Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, 142290 Pushchino, RussiaThe <i>Bacillus cereus</i> group of bacteria includes, inter alia, the species known to be associated with human diseases and food poisoning. Here, we describe the <i>Bacillus</i> phage vB_BtS_B83 (abbreviated as B83) infecting the species of this group. Transmission electron microscopy (TEM) micrographs indicate that B83 belongs to the <i>Siphoviridae</i> family. B83 is a temperate phage using an arbitrium system for the regulation of the lysis−lysogeny switch, and is probably capable of forming a circular plasmid prophage. Comparative analysis shows that it has been previously sequenced, but was mistaken for a plasmid. B83 shares common genome organization and >46% of proteins with other the <i>Bacillus</i> phage, BMBtp14. Phylograms constructed using large terminase subunits and a pan-genome presence−absence matrix show that these phages form a clade distinct from the closest viruses. Based on the above, we propose the creation of a new genus named <i>Bembunaquatrovirus</i> that includes B83 and BMBtp14.https://www.mdpi.com/1999-4915/11/7/624bacteriophagephage<i>Bacillus thuringiensis</i>plasmid prophage<i>Bembunaquatrovirus</i><i>Siphoviridae</i> |
| spellingShingle | Emma G. Piligrimova Olesya A. Kazantseva Nikita A. Nikulin Andrey M. Shadrin <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus Viruses bacteriophage phage <i>Bacillus thuringiensis</i> plasmid prophage <i>Bembunaquatrovirus</i> <i>Siphoviridae</i> |
| title | <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus |
| title_full | <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus |
| title_fullStr | <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus |
| title_full_unstemmed | <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus |
| title_short | <i>Bacillus</i> Phage vB_BtS_B83 Previously Designated as a Plasmid May Represent a New <i>Siphoviridae</i> Genus |
| title_sort | i bacillus i phage vb bts b83 previously designated as a plasmid may represent a new i siphoviridae i genus |
| topic | bacteriophage phage <i>Bacillus thuringiensis</i> plasmid prophage <i>Bembunaquatrovirus</i> <i>Siphoviridae</i> |
| url | https://www.mdpi.com/1999-4915/11/7/624 |
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