Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy
Background and Aim Regional differences in gallstone (GS) composition are well documented in the Indian subcontinent. The reasons for the same are unknown. Etiopathogenesis of GS remains elusive despite advances in instrumentation. This was an in‐depth analysis of the chemical, structural, and eleme...
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| Format: | Article |
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Wiley
2019-10-01
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| Series: | JGH Open |
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| Online Access: | https://doi.org/10.1002/jgh3.12171 |
| _version_ | 1818600060566372352 |
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| author | Mohana Bakthavatchalam Jayanthi Venkataraman Ramya J Ramana Mayank Jain Balwant Singh Arul K Thanigai Vaithiswaran Velyoudam Saravanan Manickam Neethirajan Manoj K Tiwari Ashish K Agarwal Narayana S Kalkura |
| author_facet | Mohana Bakthavatchalam Jayanthi Venkataraman Ramya J Ramana Mayank Jain Balwant Singh Arul K Thanigai Vaithiswaran Velyoudam Saravanan Manickam Neethirajan Manoj K Tiwari Ashish K Agarwal Narayana S Kalkura |
| author_sort | Mohana Bakthavatchalam |
| collection | DOAJ |
| description | Background and Aim Regional differences in gallstone (GS) composition are well documented in the Indian subcontinent. The reasons for the same are unknown. Etiopathogenesis of GS remains elusive despite advances in instrumentation. This was an in‐depth analysis of the chemical, structural, and elemental composition of GS with special reference to synchroton studies. Methods We used high‐end sensitive analytical complementary microscopic and spectroscopic methods techniques, such as X‐ray diffraction, scanning electron microscopy, Fourier transform infrared, synchrotron X‐ray fluorescence spectroscopy (SR‐XRF), and 2D and 3D synchrotron microtomography (SR‐μCT), to study the ultra structure and trace element composition of three major types of GS (cholesterol, mixed, and pigment). SR‐XRF quantified the trace elements in GS. Results The cholesterol GS (monohydrate and anhydrate) were crystalline, with high calcium content. The pigment GS were amorphous, featureless, black, and fragile, with high calcium bilirubinate and carbonate salts. They had the highest concentration of iron (average 31.50 ppm) and copper (average 92.73 ppm), with bacterial inclusion. The mixed stones had features of both cholesterol and pigment GS with intermediate levels of copper (average 20.8 ppm) and iron (average 17.78 ppm). Conclusion SR‐μCT has, for the first time, provided cross‐sectional computed imaging delineating the framework of GS and mineral distribution. It provided excellent mapping of cholesterol GS. SR‐XRF confirmed that pigment GS had high concentrations of copper and iron with bacterial inclusions, the latter possibly serving as a nidus to the formation of these stones. |
| first_indexed | 2024-12-16T12:29:29Z |
| format | Article |
| id | doaj.art-12881bf476a342fa9368439fcfc43b34 |
| institution | Directory Open Access Journal |
| issn | 2397-9070 |
| language | English |
| last_indexed | 2024-12-16T12:29:29Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | JGH Open |
| spelling | doaj.art-12881bf476a342fa9368439fcfc43b342022-12-21T22:31:43ZengWileyJGH Open2397-90702019-10-013538138710.1002/jgh3.12171Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopyMohana Bakthavatchalam0Jayanthi Venkataraman1Ramya J Ramana2Mayank Jain3Balwant Singh4Arul K Thanigai5Vaithiswaran Velyoudam6Saravanan Manickam Neethirajan7Manoj K Tiwari8Ashish K Agarwal9Narayana S Kalkura10Crystal Growth Centre Anna University Chennai IndiaInstitute of GI Sciences, Gleneagles Global Hospitals and Health City Chennai IndiaCrystal Growth Centre Anna University Chennai IndiaInstitute of GI Sciences, Gleneagles Global Hospitals and Health City Chennai IndiaIndus 2 Raja Ramanna Centre for Advance Technology Indore IndiaDepartment of Physics, Energy and Biophotonics Lab AMET Chennai IndiaInstitute of GI Sciences, Gleneagles Global Hospitals and Health City Chennai IndiaInstitute of GI Sciences, Gleneagles Global Hospitals and Health City Chennai IndiaIndus 2 Raja Ramanna Centre for Advance Technology Indore IndiaIndus 2 Raja Ramanna Centre for Advance Technology Indore IndiaCrystal Growth Centre Anna University Chennai IndiaBackground and Aim Regional differences in gallstone (GS) composition are well documented in the Indian subcontinent. The reasons for the same are unknown. Etiopathogenesis of GS remains elusive despite advances in instrumentation. This was an in‐depth analysis of the chemical, structural, and elemental composition of GS with special reference to synchroton studies. Methods We used high‐end sensitive analytical complementary microscopic and spectroscopic methods techniques, such as X‐ray diffraction, scanning electron microscopy, Fourier transform infrared, synchrotron X‐ray fluorescence spectroscopy (SR‐XRF), and 2D and 3D synchrotron microtomography (SR‐μCT), to study the ultra structure and trace element composition of three major types of GS (cholesterol, mixed, and pigment). SR‐XRF quantified the trace elements in GS. Results The cholesterol GS (monohydrate and anhydrate) were crystalline, with high calcium content. The pigment GS were amorphous, featureless, black, and fragile, with high calcium bilirubinate and carbonate salts. They had the highest concentration of iron (average 31.50 ppm) and copper (average 92.73 ppm), with bacterial inclusion. The mixed stones had features of both cholesterol and pigment GS with intermediate levels of copper (average 20.8 ppm) and iron (average 17.78 ppm). Conclusion SR‐μCT has, for the first time, provided cross‐sectional computed imaging delineating the framework of GS and mineral distribution. It provided excellent mapping of cholesterol GS. SR‐XRF confirmed that pigment GS had high concentrations of copper and iron with bacterial inclusions, the latter possibly serving as a nidus to the formation of these stones.https://doi.org/10.1002/jgh3.12171cholesterolelementalgallstonesmicrotomographypigmentsynchroton |
| spellingShingle | Mohana Bakthavatchalam Jayanthi Venkataraman Ramya J Ramana Mayank Jain Balwant Singh Arul K Thanigai Vaithiswaran Velyoudam Saravanan Manickam Neethirajan Manoj K Tiwari Ashish K Agarwal Narayana S Kalkura Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy JGH Open cholesterol elemental gallstones microtomography pigment synchroton |
| title | Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy |
| title_full | Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy |
| title_fullStr | Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy |
| title_full_unstemmed | Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy |
| title_short | Morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron X‐ray fluorescence spectroscopy |
| title_sort | morphological and elemental mapping of gallstones using synchrotron microtomography and synchrotron x ray fluorescence spectroscopy |
| topic | cholesterol elemental gallstones microtomography pigment synchroton |
| url | https://doi.org/10.1002/jgh3.12171 |
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