Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus
Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzyme...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
1998-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://www.pressesagro.be/base/text/v2n2/128.pdf |
| _version_ | 1818561775527788544 |
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| author | Baudoin JP. Fofana B. Berken B. Hardy O. Vekemans X., |
| author_facet | Baudoin JP. Fofana B. Berken B. Hardy O. Vekemans X., |
| author_sort | Baudoin JP. |
| collection | DOAJ |
| description | Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzymes. Thirty-six haplotypes are identified on the basis of the polymorphism in fragment number and size. Three main phylogenetic groups, strongly supported through bootstrap analysis, are identified: (1) accessions from Phaseolus lunatus and Phaseolus xolocotzii; (2) accessions from Phaseolus glabellus; (3) accessions from Phaseolus vulgaris, Phaseolus polyanthus and Phaseolus coccineus. Within the third group, accessions of Phaseolus coccineus are scattered along the phylogenetic tree, which provides some evidence that coccineus accessions are paraphyletic with respect to Phaseolus vulgaris and Phaseolus polyanthus. An analysis of molecular variance applied on four species show that they are significantly differentiated with 79% of molecular variance among species and 21% within species. The results agree with previous investigations on chloroplast DNA variation in the genus Phaseolus, and suggest that PCRRFLP methods, which are technically less labour-intensive than previous methods, are of great value for phylogenetic investigations at the generic level. |
| first_indexed | 2024-12-14T00:55:09Z |
| format | Article |
| id | doaj.art-128f08a0ca4445afb43012883cc92c00 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-12-14T00:55:09Z |
| publishDate | 1998-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-128f08a0ca4445afb43012883cc92c002022-12-21T23:23:36ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45071998-01-0122128134Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre PhaseolusBaudoin JP.Fofana B.Berken B.Hardy O.Vekemans X.,Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzymes. Thirty-six haplotypes are identified on the basis of the polymorphism in fragment number and size. Three main phylogenetic groups, strongly supported through bootstrap analysis, are identified: (1) accessions from Phaseolus lunatus and Phaseolus xolocotzii; (2) accessions from Phaseolus glabellus; (3) accessions from Phaseolus vulgaris, Phaseolus polyanthus and Phaseolus coccineus. Within the third group, accessions of Phaseolus coccineus are scattered along the phylogenetic tree, which provides some evidence that coccineus accessions are paraphyletic with respect to Phaseolus vulgaris and Phaseolus polyanthus. An analysis of molecular variance applied on four species show that they are significantly differentiated with 79% of molecular variance among species and 21% within species. The results agree with previous investigations on chloroplast DNA variation in the genus Phaseolus, and suggest that PCRRFLP methods, which are technically less labour-intensive than previous methods, are of great value for phylogenetic investigations at the generic level.http://www.pressesagro.be/base/text/v2n2/128.pdfPhaseolusphylogenychloroplast DNAPCRRFLPplant genetic resources |
| spellingShingle | Baudoin JP. Fofana B. Berken B. Hardy O. Vekemans X., Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus Biotechnologie, Agronomie, Société et Environnement Phaseolus phylogeny chloroplast DNA PCR RFLP plant genetic resources |
| title | Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus |
| title_full | Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus |
| title_fullStr | Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus |
| title_full_unstemmed | Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus |
| title_short | Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus |
| title_sort | utilisation de la pcr rflp sur de l adn chloro plastique pour l etude des relations phylogenetiques au sein du genre phaseolus |
| topic | Phaseolus phylogeny chloroplast DNA PCR RFLP plant genetic resources |
| url | http://www.pressesagro.be/base/text/v2n2/128.pdf |
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