Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Chemical Engineers of Serbia
2015-01-01
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| Series: | Hemijska Industrija |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2015/0367-598X1400021K.pdf |
| Summary: | Despite the fact that the methods based on the osmotic properties of the
cells are the most widely used for loading of drugs in human and animal
erythrocytes, data related to the osmotic properties of erythrocytes derived
from animal blood are scarce. This work was performed with an aim to
investigate the possibility of use the flow cytometry as a tool for
determination the osmotic behaviour of porcine and bovine erythrocytes, and
thus facilitate the engineering of erythrocytes from animal blood to be drug
carriers. The method of flow cytometry successfully provided the information
about bovine and porcine erythrocyte osmotic fragility, and made the initial
steps in assessment of erythrocyte shape in a large number of erythrocytes.
Although this method is not able to confirm the swelling of pig erythrocytes,
it indicated to the differences in pig erythrocytes that had basic
hematological parameters inside and outside the reference values. In order to
apply/use the porcine and bovine erythrocytes as drug carriers, the method of
flow cytometry, confirming the presence of osmotically different fractions of
red blood cells, indicated that various amounts of the encapsulated drug in
porcine and bovine erythrocytes can be expected. |
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| ISSN: | 0367-598X 2217-7426 |