Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i>
Heather honey was tested for its effect on the formation of biofilms by <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Enterococcus faecalis</i>, <i>Salmonella En...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/12/911 |
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| author | Oscar Shirlaw Zara Billah Baraa Attar Lisa Hughes Rana M. Qasaymeh Veronique Seidel Georgios Efthimiou |
| author_facet | Oscar Shirlaw Zara Billah Baraa Attar Lisa Hughes Rana M. Qasaymeh Veronique Seidel Georgios Efthimiou |
| author_sort | Oscar Shirlaw |
| collection | DOAJ |
| description | Heather honey was tested for its effect on the formation of biofilms by <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Enterococcus faecalis</i>, <i>Salmonella Enteriditis</i> and <i>Acinetobacter baumanii</i> in comparison with Manuka honey. At 0.25 mg/mL, Heather honey inhibited biofilm formation in <i>S. aureus</i>, <i>A. baumanii</i>, <i>E. coli</i>, <i>S. Enteriditis</i> and <i>P. aeruginosa</i>, but promoted the growth of <i>E. faecalis</i> and <i>K. pneumoniae</i> biofilms. Manuka honey inhibited biofilm formation in <i>K. pneumoniae</i>, <i>E. faecalis</i>, and <i>S. Enteriditis</i>, <i>A. baumanii</i>, <i>E. coli</i> and <i>P. aeruginosa</i>, but promoted <i>S. aureus</i> biofilm formation. Molecular docking with Autodock Vina was performed to calculate the predictive binding affinities and ligand efficiencies of Manuka and Heather honey constituents for PaDsbA1, the main enzyme controlling the correct folding of virulence proteins in <i>Pseudomonas aeruginosa</i>. A number of constituents, including benzoic acid and methylglyoxal, present in Heather and/or Manuka honey, revealed high ligand efficiencies for the target enzyme. This helps support, to some extent, the decrease in <i>P. aeruginosa</i> biofilm formation observed for such honeys. |
| first_indexed | 2024-03-10T14:02:39Z |
| format | Article |
| id | doaj.art-128fba350d72424b888ee4f4d5b93263 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T14:02:39Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-128fba350d72424b888ee4f4d5b932632023-11-21T00:56:39ZengMDPI AGAntibiotics2079-63822020-12-0191291110.3390/antibiotics9120911Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i>Oscar Shirlaw0Zara Billah1Baraa Attar2Lisa Hughes3Rana M. Qasaymeh4Veronique Seidel5Georgios Efthimiou6Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UKDepartment of Biomedical and Forensic Sciences, Hardy Building, University of Hull, Hull HU6 7RX, UKHeather honey was tested for its effect on the formation of biofilms by <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Enterococcus faecalis</i>, <i>Salmonella Enteriditis</i> and <i>Acinetobacter baumanii</i> in comparison with Manuka honey. At 0.25 mg/mL, Heather honey inhibited biofilm formation in <i>S. aureus</i>, <i>A. baumanii</i>, <i>E. coli</i>, <i>S. Enteriditis</i> and <i>P. aeruginosa</i>, but promoted the growth of <i>E. faecalis</i> and <i>K. pneumoniae</i> biofilms. Manuka honey inhibited biofilm formation in <i>K. pneumoniae</i>, <i>E. faecalis</i>, and <i>S. Enteriditis</i>, <i>A. baumanii</i>, <i>E. coli</i> and <i>P. aeruginosa</i>, but promoted <i>S. aureus</i> biofilm formation. Molecular docking with Autodock Vina was performed to calculate the predictive binding affinities and ligand efficiencies of Manuka and Heather honey constituents for PaDsbA1, the main enzyme controlling the correct folding of virulence proteins in <i>Pseudomonas aeruginosa</i>. A number of constituents, including benzoic acid and methylglyoxal, present in Heather and/or Manuka honey, revealed high ligand efficiencies for the target enzyme. This helps support, to some extent, the decrease in <i>P. aeruginosa</i> biofilm formation observed for such honeys.https://www.mdpi.com/2079-6382/9/12/911antibiofilmantivirulenceHeather honeyManuka honey<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Oscar Shirlaw Zara Billah Baraa Attar Lisa Hughes Rana M. Qasaymeh Veronique Seidel Georgios Efthimiou Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> Antibiotics antibiofilm antivirulence Heather honey Manuka honey <i>Pseudomonas aeruginosa</i> |
| title | Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> |
| title_full | Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> |
| title_short | Antibiofilm Activity of Heather and Manuka Honeys and Antivirulence Potential of Some of Their Constituents on the DsbA1 Enzyme of <i>Pseudomonas aeruginosa</i> |
| title_sort | antibiofilm activity of heather and manuka honeys and antivirulence potential of some of their constituents on the dsba1 enzyme of i pseudomonas aeruginosa i |
| topic | antibiofilm antivirulence Heather honey Manuka honey <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/2079-6382/9/12/911 |
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