Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509
During the conversion of lignocellulose, phenolic wastewaters are generated. Therefore, researchers have investigated wastewater valorization processes in which these pollutants are converted to chemicals, i.e., lipids. However, wastewaters are lean feedstocks, so these valorization processes in res...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/5/204 |
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| author | Waut Broos Nikolett Wittner Jordi Geerts Jan Dries Siegfried E. Vlaeminck Nina Gunde-Cimerman Aurore Richel Iris Cornet |
| author_facet | Waut Broos Nikolett Wittner Jordi Geerts Jan Dries Siegfried E. Vlaeminck Nina Gunde-Cimerman Aurore Richel Iris Cornet |
| author_sort | Waut Broos |
| collection | DOAJ |
| description | During the conversion of lignocellulose, phenolic wastewaters are generated. Therefore, researchers have investigated wastewater valorization processes in which these pollutants are converted to chemicals, i.e., lipids. However, wastewaters are lean feedstocks, so these valorization processes in research typically require the addition of large quantities of sugars and sterilization, which increase costs. This paper investigates a repeated batch fermentation strategy with <i>Rhodotorula kratochvilovae</i> EXF7516 and <i>Cutaneotrichosporon oleaginosum</i> ATCC 20509, without these requirements. The pollutant removal and its conversion to microbial oil were evaluated. Because of the presence of non-monomeric substrates, the ligninolytic enzyme activity was also investigated. The repeated batch fermentation strategy was successful, as more lipids accumulated every cycle, up to a total of 5.4 g/L (23% cell dry weight). In addition, the yeasts consumed up to 87% of monomeric substrates, i.e., sugars, aromatics, and organics acids, and up to 23% of non-monomeric substrates, i.e., partially degraded xylan, lignin, cellulose. Interestingly, lipid production was only observed during the harvest phase of each cycle, as the cells experienced stress, possibly due to oxygen limitation. This work presents the first results on the feasibility of valorizing non-sterilized lignocellulosic wastewater with <i>R. kratochvilovae</i> and <i>C. oleaginosum</i> using a cost-effective repeated batch strategy. |
| first_indexed | 2024-03-10T03:54:47Z |
| format | Article |
| id | doaj.art-128fc4aa046b489aaa996ee415916089 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T03:54:47Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-128fc4aa046b489aaa996ee4159160892023-11-23T10:56:56ZengMDPI AGFermentation2311-56372022-04-018520410.3390/fermentation8050204Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509Waut Broos0Nikolett Wittner1Jordi Geerts2Jan Dries3Siegfried E. Vlaeminck4Nina Gunde-Cimerman5Aurore Richel6Iris Cornet7Faculty of Applied Engineering, BioWAVE, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumFaculty of Applied Engineering, BioWAVE, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumFaculty of Applied Engineering, BioWAVE, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumFaculty of Applied Engineering, BioWAVE, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumDepartment of Bioscience Engineering, Sustainable Energy, Air and Water Technology, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaGembloux Agro-Bio Tech, Université Liège, Passage des Déportés 2, 5030 Gembloux, BelgiumFaculty of Applied Engineering, BioWAVE, Universiteit Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, BelgiumDuring the conversion of lignocellulose, phenolic wastewaters are generated. Therefore, researchers have investigated wastewater valorization processes in which these pollutants are converted to chemicals, i.e., lipids. However, wastewaters are lean feedstocks, so these valorization processes in research typically require the addition of large quantities of sugars and sterilization, which increase costs. This paper investigates a repeated batch fermentation strategy with <i>Rhodotorula kratochvilovae</i> EXF7516 and <i>Cutaneotrichosporon oleaginosum</i> ATCC 20509, without these requirements. The pollutant removal and its conversion to microbial oil were evaluated. Because of the presence of non-monomeric substrates, the ligninolytic enzyme activity was also investigated. The repeated batch fermentation strategy was successful, as more lipids accumulated every cycle, up to a total of 5.4 g/L (23% cell dry weight). In addition, the yeasts consumed up to 87% of monomeric substrates, i.e., sugars, aromatics, and organics acids, and up to 23% of non-monomeric substrates, i.e., partially degraded xylan, lignin, cellulose. Interestingly, lipid production was only observed during the harvest phase of each cycle, as the cells experienced stress, possibly due to oxygen limitation. This work presents the first results on the feasibility of valorizing non-sterilized lignocellulosic wastewater with <i>R. kratochvilovae</i> and <i>C. oleaginosum</i> using a cost-effective repeated batch strategy.https://www.mdpi.com/2311-5637/8/5/204microbial oilwastewaterlignocelluloseyeasttriacylglyceridesunsterile fermentation |
| spellingShingle | Waut Broos Nikolett Wittner Jordi Geerts Jan Dries Siegfried E. Vlaeminck Nina Gunde-Cimerman Aurore Richel Iris Cornet Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 Fermentation microbial oil wastewater lignocellulose yeast triacylglycerides unsterile fermentation |
| title | Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 |
| title_full | Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 |
| title_fullStr | Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 |
| title_full_unstemmed | Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 |
| title_short | Evaluation of Lignocellulosic Wastewater Valorization with the Oleaginous Yeasts <i>R. kratochvilovae</i> EXF7516 and <i>C. oleaginosum</i> ATCC 20509 |
| title_sort | evaluation of lignocellulosic wastewater valorization with the oleaginous yeasts i r kratochvilovae i exf7516 and i c oleaginosum i atcc 20509 |
| topic | microbial oil wastewater lignocellulose yeast triacylglycerides unsterile fermentation |
| url | https://www.mdpi.com/2311-5637/8/5/204 |
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