Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i>
The yeast <i>Cyberlindnera jadinii</i> has great potential in the biotechnology industry due to its ability to produce a variety of compounds of interest, including carboxylic acids. In this work, we identified genes encoding carboxylate transporters from this yeast species. The function...
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MDPI AG
2022-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/1/51 |
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| author | Maria Sousa-Silva Pedro Soares João Alves Daniel Vieira Margarida Casal Isabel Soares-Silva |
| author_facet | Maria Sousa-Silva Pedro Soares João Alves Daniel Vieira Margarida Casal Isabel Soares-Silva |
| author_sort | Maria Sousa-Silva |
| collection | DOAJ |
| description | The yeast <i>Cyberlindnera jadinii</i> has great potential in the biotechnology industry due to its ability to produce a variety of compounds of interest, including carboxylic acids. In this work, we identified genes encoding carboxylate transporters from this yeast species. The functional characterization of sixteen plasma membrane carboxylate transporters belonging to the AceTr, SHS, TDT, MCT, SSS, and DASS families was performed by heterologous expression in <i>Saccharomyces cerevisiae</i>. The newly identified <i>C. jadinii</i> transporters present specificity for mono-, di-, and tricarboxylates. The transporters CjAto5, CjJen6, CjSlc5, and CjSlc13-1 display the broadest substrate specificity; CjAto2 accepts mono- and dicarboxylates; and CjAto1,3,4, CjJen1-5, CjSlc16, and CjSlc13-2 are specific for monocarboxylic acids. A detailed characterization of these transporters, including phylogenetic reconstruction, 3D structure prediction, and molecular docking analysis is presented here. The properties presented by these transporters make them interesting targets to be explored as organic acid exporters in microbial cell factories. |
| first_indexed | 2024-03-10T01:09:57Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T01:09:57Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-129048ef69814fe792ae7eb6dd72a53f2023-11-23T14:18:13ZengMDPI AGJournal of Fungi2309-608X2022-01-01815110.3390/jof8010051Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i>Maria Sousa-Silva0Pedro Soares1João Alves2Daniel Vieira3Margarida Casal4Isabel Soares-Silva5Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalCentre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalThe yeast <i>Cyberlindnera jadinii</i> has great potential in the biotechnology industry due to its ability to produce a variety of compounds of interest, including carboxylic acids. In this work, we identified genes encoding carboxylate transporters from this yeast species. The functional characterization of sixteen plasma membrane carboxylate transporters belonging to the AceTr, SHS, TDT, MCT, SSS, and DASS families was performed by heterologous expression in <i>Saccharomyces cerevisiae</i>. The newly identified <i>C. jadinii</i> transporters present specificity for mono-, di-, and tricarboxylates. The transporters CjAto5, CjJen6, CjSlc5, and CjSlc13-1 display the broadest substrate specificity; CjAto2 accepts mono- and dicarboxylates; and CjAto1,3,4, CjJen1-5, CjSlc16, and CjSlc13-2 are specific for monocarboxylic acids. A detailed characterization of these transporters, including phylogenetic reconstruction, 3D structure prediction, and molecular docking analysis is presented here. The properties presented by these transporters make them interesting targets to be explored as organic acid exporters in microbial cell factories.https://www.mdpi.com/2309-608X/8/1/51<i>Cyberlindnera jadinii</i>carboxylic acidsplasma membrane transportersphylogenyprotein structure predictionmolecular docking |
| spellingShingle | Maria Sousa-Silva Pedro Soares João Alves Daniel Vieira Margarida Casal Isabel Soares-Silva Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> Journal of Fungi <i>Cyberlindnera jadinii</i> carboxylic acids plasma membrane transporters phylogeny protein structure prediction molecular docking |
| title | Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> |
| title_full | Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> |
| title_fullStr | Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> |
| title_full_unstemmed | Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> |
| title_short | Uncovering Novel Plasma Membrane Carboxylate Transporters in the Yeast <i>Cyberlindnera jadinii</i> |
| title_sort | uncovering novel plasma membrane carboxylate transporters in the yeast i cyberlindnera jadinii i |
| topic | <i>Cyberlindnera jadinii</i> carboxylic acids plasma membrane transporters phylogeny protein structure prediction molecular docking |
| url | https://www.mdpi.com/2309-608X/8/1/51 |
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